Application
Western blot analysis in HL-60 cell line lysates (35ug/lane).Application
Flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | TNF Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Tumor necrosis factor, Cachectin, TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNF-a, Tumor necrosis factor, membrane form, N-terminal fragment, NTF, Intracellular domain 1, ICD1, Intracellular domain 2, ICD2, C-domain 1, C-domain 2, Tumor |
| Product description | This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine.1) Majewski, P.M., et al. J. Biol. Chem. 285(45):34828-34838(2010) |
| Immunogen | This TNF antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 187-216 amino acids from the C-terminal region of human TNF. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSWB |
| For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TNF |
|---|---|
| Alternative Names | Tumor necrosis factor Cachectin TNF-alpha Tumor necrosis factor ligand superfamily member 2 TNF-a Tumor necrosis factor membrane form N-terminal fragment NTF Intracellular domain 1 ICD1 Intracellular domain 2 ICD2 C-domain 1 C-domain 2 Tumor |
| Molecular Weight(MW) | 26 kDa |
Application
Western blot analysis in HL-60 cell line lysates (35ug/lane).
Application
Flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
Data sheet for OM293627