Application
To detect Human TNF-alpha by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci’s Biotinylated Anti-Human TNF-alpha (XP-5282Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human TNF-alpha.Application
This antibody stained formalin-fixed, paraffin-embedded sections of human breast invasive ductal carcinoma. The recommended concentrations are 0.5 ug/ml-1.0 ug/ml with an overnight incubation at 4˚C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 Sodium Citrate buffer is recommended. Optimal concentrations and conditions may| Product Name | TNF-a Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | DIF, TNFA, TNFSF2, TNF-alpha, Tumor necrosis factor, Cachectin, TNF-a |
| Product description | Tumor Necrosis Factor Alpha (TNF alpha) is a protein secreted by lipopolysaccharide stimulated macrophages, and causes tumor necrosis when injected into tumour bearing mice. TNF alpha is believed to mediate pathogenic shock and tissue injury associated with endotoxemia. |
| Immunogen | Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hTNF-alpha (human Tumor Necrosis Factor-alpha). |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of hTNF-α (0.25 ng/mL), a concentration of 0.012 - 0.014 μg/mL of this antibody is required. ELISA: To detect hTNF-α by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hTNF-α. Sandwich: To detect hTNF-α by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human TNF-α (XP-5282Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hTNF-α. Western Blot: To detect hTNF-α by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hTNF-α is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.: |
|
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TNF |
|---|---|
| Alternative Names | DIF TNFA TNFSF2 TNF-alpha Tumor necrosis factor Cachectin TNF-a |
Application
To detect Human TNF-alpha by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci’s Biotinylated Anti-Human TNF-alpha (XP-5282Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human TNF-alpha.
Application
This antibody stained formalin-fixed, paraffin-embedded sections of human breast invasive ductal carcinoma. The recommended concentrations are 0.5 ug/ml-1.0 ug/ml with an overnight incubation at 4˚C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 Sodium Citrate buffer is recommended. Optimal concentrations and conditions may| Application Notes | Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of hTNF-α (0.25 ng/mL), a concentration of 0.012 - 0.014 μg/mL of this antibody is required. ELISA: To detect hTNF-α by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hTNF-α. Sandwich: To detect hTNF-α by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human TNF-α (XP-5282Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hTNF-α. Western Blot: To detect hTNF-α by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hTNF-α is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | TNF-a antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Data sheet for OM293631