Application
To detect Rat TNF-alpha by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci’s Biotinylated Anti-Rat TNF-alpha (XP-5286Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat TNF-alpha.Application
This antibody stained colchicine injected rat brain (hippocampus CA1 region) tissue. The primary antibody was incubated at 1.0 ug/ml overnight at 4˚C. This was followed by a peroxidase conjugated secondary antibody and then a fluorescein Tyramide Signal Amplification (TSA) reagent. Optimal concentrations and conditions may vary.| Product Name | TNF-a Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Tnfa, RATTNF, TNF-alpha, Tnfa, Tnfsf2, Tumor necrosis factor, Cachectin, TNF-a |
| Product description | Tumor Necrosis Factor Alpha (TNF alpha) is a protein secreted by lipopolysaccharide stimulated macrophages, and causes tumor necrosis when injected into tumour bearing mice. TNF alpha is believed to mediate pathogenic shock and tissue injury associated with endotoxemia. |
| Immunogen | Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant Rat TNF alpha. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISANeutWB |
| Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of Rat TNF alpha (2.0 ng/mL), a concentration of 0.145 - 0.165 μg/mL of this antibody is required. ELISA: To detect Rat TNF alpha by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant Rat TNF alpha. Sandwich: To detect Rat TNF-α by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Rat TNF-α (XP-5286Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat TNF-α. Western Blot: To detect Rat TNF alpha by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant Rat TNF alpha is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.: |
|
| Species Reactivity | Rat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | Tnf |
|---|---|
| Alternative Names | Tnfa RATTNF TNF-alpha Tnfa Tnfsf2 Tumor necrosis factor Cachectin TNF-a |
Application
To detect Rat TNF-alpha by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci’s Biotinylated Anti-Rat TNF-alpha (XP-5286Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat TNF-alpha.
Application
This antibody stained colchicine injected rat brain (hippocampus CA1 region) tissue. The primary antibody was incubated at 1.0 ug/ml overnight at 4˚C. This was followed by a peroxidase conjugated secondary antibody and then a fluorescein Tyramide Signal Amplification (TSA) reagent. Optimal concentrations and conditions may vary.| Application Notes | Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of Rat TNF alpha (2.0 ng/mL), a concentration of 0.145 - 0.165 μg/mL of this antibody is required. ELISA: To detect Rat TNF alpha by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant Rat TNF alpha. Sandwich: To detect Rat TNF-α by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Rat TNF-α (XP-5286Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat TNF-α. Western Blot: To detect Rat TNF alpha by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant Rat TNF alpha is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | TNF-a antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
