Application
Western blot analysis of TNFAIP3 in SK-N-SH cell lysate with TNFAIP3 antibody at 1 μg/mL.Application
Immunofluorescence of TNFAIP3 in Human Lung cells with TNFAIP3 antibody at 20 μg/mL.Application
Immunohistochemistry of TNFAIP3 in human lung tissue with TNFAIP3 antibody at 5 μg/mL.| Product Name | TNFAIP3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | TNFAIP3 Antibody: TNFAIP3, also known as A20, is located in chromosome band 6q23, a region that is often deleted in B cell lymphomas. Recently, it was identified as a tumor suppressor gene in Hodgkin lymphoma and several subtypes of non-Hodgkin lymphomas. TNFAIP3 was initially identified as a zinc-finger protein that is rapidly and transiently induced by TNF-α, inhibiting NF-κB-dependent gene expression, and protecting cells from TNF-α-cytotoxicity. Overexpression of TNFAIP3 also inhibits the TLR2- and TLR4-mediated interleukin-8 synthesis in airway epithelial cells, suggesting that TNFAIP3 also acts as a negative regulator of TLR-mediated inflammatory responses, thereby protecting the host against harmful over-responses to pathogens. At least two isoforms of TNFAIP3 are known to exist.1) Zhang YP, Matthiesen S, Harder R, et al. A 3-cM commonly deleted region in 6q21 in leukemias and lymphomas delineated by fluorescence in situ hybridization. Genes Chromosomes Cancer2000; 27:52-58. |
| Immunogen | TNFAIP3 antibody was raised against a 14 amino acid synthetic peptide near the carboxy terminus of human TNFAIP3. The immunogen is located within the last 50 amino acids of TNFAIP3. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| TNFAIP3 antibody can be used for detection of TNFAIP3 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | Tnfaip3 |
|---|---|
| Alternative Names | TNFAIP3 Antibody: A20 Tnfip3 Tumor necrosis factor alpha-induced protein 3 Putative DNA-binding protein A20 TNF alpha-induced protein 3 |
Application
Western blot analysis of TNFAIP3 in SK-N-SH cell lysate with TNFAIP3 antibody at 1 μg/mL.
Application
Immunofluorescence of TNFAIP3 in Human Lung cells with TNFAIP3 antibody at 20 μg/mL.
Application
Immunohistochemistry of TNFAIP3 in human lung tissue with TNFAIP3 antibody at 5 μg/mL.| Positive Control | 1) Cat. No. 1210 - HEK293 Cell Lysate |
|---|---|
| Application Notes | TNFAIP3 antibody can be used for detection of TNFAIP3 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | TNFAIP3 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | TNFAIP3 Antibody is supplied in PBS containing 0.02% sodium azide. |
