Application
Western blot analysis of TNFSF4 in rat spleen tissue lysate with TNFSF4 antibody at (A) 0.5 and (B) 1 μg/mLApplication
Immunofluorescence of TNFSF4 in human spleen tissue with TNFSF4 antibody at 20 μg/ml.Application
Immunohistochemistry of TNFSF4 in spleen tissue with TNFSF4 antibody at 5 μg/ml.| Product Name | TNFSF4 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | TNFSF4 Antibody: GP34, CD252, OX4OL, TXGP1, CD134L, OX-40L, Tumor necrosis factor ligand superfamily member 4, Glycoprotein Gp34, OX40L |
| Product description | TNFSF4 Antibody: The tumor necrosis factor superfamily member TNFSF4 is a type II membrane bound, non-covalently linked homotrimeric protein. It is expressed on antigen presenting cells, such as dendritic cells and activated B-cells, and also on other cells such as vascular endothelial cells, mast cells, and natural killer cells. TNFSF4 signals specifically through the TNFRSF4 receptor, is expressed predominantly on CD4+T cells but also on certain activated CD8+T cells. TNFRSF4/TNFSF4 functions as a costimulatory signal, which is required for a productive interaction between antigen presenting cells and their target T-cells. It enhances cell proliferation and survival, and increases expression of RANTES, IL-2, IL-3, and IFNγ. TNFRSF4/TNFSF4 signaling plays an important role in immunotolerance.1) Arch RH and Thompson CB. 4-1BB and Ox40 are members of a tumor necrosis factor (TNF)-nerve growth factor receptor subfamily that bind TNF receptor-associated factors and activate nuclear factor kappaB. Mol. Cell Biol.1998; 18:558-65. |
| Immunogen | Rabbit polyclonal TNFSF4 antibody was raised against a 14 amino acid peptide near the carboxy terminus of human TNFSF4. The immunogen is located within the last 50 amino acids of TNFSF4. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| TNFSF4 antibody can be used for detection of TNFSF4 by Western blot at 0.5 - 1 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TNFSF4 |
|---|---|
| Alternative Names | TNFSF4 Antibody: GP34 CD252 OX4OL TXGP1 CD134L OX-40L Tumor necrosis factor ligand superfamily member 4 Glycoprotein Gp34 OX40L |
| Molecular Weight(MW) | 20 kDa |
| Sequence Similarities | 2) Cat. No. 10-901 - Human Spleen Tissue Slide |
| Tissue Specificity | At least two isoforms of TNFSF4 are known to exist; this antibody will detect both isoforms. |
Application
Western blot analysis of TNFSF4 in rat spleen tissue lysate with TNFSF4 antibody at (A) 0.5 and (B) 1 μg/mL
Application
Immunofluorescence of TNFSF4 in human spleen tissue with TNFSF4 antibody at 20 μg/ml.
Application
Immunohistochemistry of TNFSF4 in spleen tissue with TNFSF4 antibody at 5 μg/ml.| Positive Control | 1) Cat. No. 1466 - Rat Spleen Tissue Lysate |
|---|---|
| Application Notes | TNFSF4 antibody can be used for detection of TNFSF4 by Western blot at 0.5 - 1 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | TNFSF4 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | TNFSF4 Antibody is supplied in PBS containing 0.02% sodium azide. |
