WB
Western blot analysis of OX40L/TNFSF4 using anti-OX40L/TNFSF4 antibody. Lane 1: recombinant mouse CD252 protein 1ng. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-OX40L/TNFSF4 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate. A specific band was detected for OX40L/TNFSF4 at approximately 21-25 kDa.IHC
IHC analysis of OX40L/TNFSF4 using anti-OX40L/TNFSF4 antibody. OX40L/TNFSF4 was detected in a paraffin-embedded section of rat small intestine tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-OX40L/TNFSF4 Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.| Product Name | Rabbit polyclonal antibody to OX40L/TNFSF4 |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E. coli-derived mouse CD252 recombinant protein (Position: Q49-L198). Mouse CD252 shares 42.7% and 78.8% amino acid (aa) sequence identity with human and rat CD252, respectively. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| WB:1:500-1:2000 IHC:1:50-1:400 |
|
| Species Reactivity | MouseRat |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TNFSF4 |
|---|---|
| Gene Synonyms | GP34 CD252 OX4OL TXGP1 CD134L OX-40L TNLG2B |
| Gene Full Name | TNF superfamily member 4 |
| Gene Summary | This gene encodes a cytokine of the tumor necrosis factor (TNF) ligand family. The encoded protein functions in T cell antigen-presenting cell (APC) interactions and mediates adhesion of activated T cells to endothelial cells. Polymorphisms in this gene have been associated with Sjogren's syndrome and systemic lupus erythematosus. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014] |
| Alternative Names | TNF superfamily member 4 CD134L CD252 GP34 OX-40L OX4OL TNLG2B TXGP1 Tnfsf4 |
| Molecular Weight(MW) | 22kDa |
| Cellular Localization | Membrane. |
WB
Western blot analysis of OX40L/TNFSF4 using anti-OX40L/TNFSF4 antibody. Lane 1: recombinant mouse CD252 protein 1ng. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-OX40L/TNFSF4 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate. A specific band was detected for OX40L/TNFSF4 at approximately 21-25 kDa.
IHC
IHC analysis of OX40L/TNFSF4 using anti-OX40L/TNFSF4 antibody. OX40L/TNFSF4 was detected in a paraffin-embedded section of rat small intestine tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-OX40L/TNFSF4 Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.| Application Notes | WB:1:500-1:2000 IHC:1:50-1:400 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| Storage Buffer | 500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
