Application
Western blot analysis of extracts from HeLa cells (Lane 2), A549 cells (Lane 3) and HepG2 cells (Lane 4), using TOB1 (Phospho-Ser164) Antibody. The lane on the left is treated with antigen-specific peptide.Application
Immunohistochemical analysis of paraffin-embedded human brain tissue using TOB1 (Phospho-Ser164) antibody (left) or the same antibody preincubated with blocking peptide (right).| Product Name | TOB1 (phospho Ser164) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | TOB, Transducer of erbB-2 1 |
| Product description | This gene encodes a member of the tob/btg1 family of anti-proliferative proteins that have the potential to regulate cell growth. When exogenously expressed, this protein supresses cell growth in tissue culture. The protein undergoes phophorylation by a serine/threonine kinase, 90 kDa ribosomal S6 kinase. Interactions of this protein with the v-erb-b2 erythroblastic leukemia viral oncogene homolog 2 gene product p185 interferes with growth suppression. This protein inhibits T cell proliferation and transcription of cytokines and cyclins. The protein interacts with both mothers against decapentaplegic Drosophila homolog 2 and 4 to enhance their DNA binding activity. This interaction inhibits interleukin 2 transcription in T cells.1) Matsuda S., Oncogene 12:705-713(1996). |
| Immunogen | TOB1 (phospho Ser164) antibody was raised against a peptide sequence around phosphorylation site of Serine 164 (A-V-S (p)-P-T) derived from Human TOB1. |
| Modification | p-Ser164 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TOB1 |
|---|---|
| Alternative Names | TOB Transducer of erbB-2 1 |
| Molecular Weight(MW) | 40 kDa |
| Tissue Specificity | The antibody detects endogenous levels of TOB1 only when phosphorylated at serine 164. |
| Entrez Gene | 10140 |
|---|---|
| Protein Accession | NP_001230806.1 |
Application
Western blot analysis of extracts from HeLa cells (Lane 2), A549 cells (Lane 3) and HepG2 cells (Lane 4), using TOB1 (Phospho-Ser164) Antibody. The lane on the left is treated with antigen-specific peptide.
Application
Immunohistochemical analysis of paraffin-embedded human brain tissue using TOB1 (Phospho-Ser164) antibody (left) or the same antibody preincubated with blocking peptide (right).| Application Notes | Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
