Application
Immunofluorescence of TOCA-1 in Human Brain cells with TOCA-1 antibody at 20 μg/mL.| Product Name | TOCA-1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | TOCA-1 Antibody: TOCA1, C1orf39, TOCA1, Formin-binding protein 1-like, Transducer of Cdc42-dependent actin assembly protein 1, Toca-1 |
| Product description | TOCA-1 Antibody: Actin reorganization is important for the regulation of neuronal morphology. A protein involved in this process, the transducer of cdc42-dependent actin assembly 1 (TOCA-1) protein, a member of the evolutionarily conserved pombe CDC15 homology (PCH) protein family, is an essential component of the Cdc42 pathway. TOCA-1 binds both N-WASP and Cdc42 and is essential for Cdc42- and PIP2-induced actin polymerization, suggesting that TOCA-1 mediates Cdc42-dependent actin nucleation by activating the N-WASP-WIP complex. Decreased expression of TOCA-1 significantly enhances neurite elongation in PC-12 cells; its overexpression in the same cells suppresses neurite elongation. It has been suggested that TOCA-1 negatively regulates axon branching by regulating membrane trafficking by regulating membrane trafficking through the F-BAR/EFC domain. Multiple isoforms of TOCA-1 are known to exist.1) Ho H-Y H, Rohatgi R, Lebensohn AM, et al. Toca-1 mediates Cdc42-dependent actin nucleation by activating the N-WASP-WIP complex. Cell2004; 118:203-16. |
| Immunogen | TOCA-1 antibody was raised against a 15 amino acid synthetic peptide near the amino terminus of the human TOCA-1. The immunogen is located within amino acids 40 - 90 of TOCA-1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IFIHC-P |
| TOCA-1 antibody can be used for detection of TOCA-1 by Western blot at 0.5 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | Rat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | FNBP1L |
|---|---|
| Alternative Names | TOCA-1 Antibody: TOCA1 C1orf39 TOCA1 Formin-binding protein 1-like Transducer of Cdc42-dependent actin assembly protein 1 Toca-1 |
| Sequence Similarities | 2) Cat. No. 10-301 - Human Brain Tissue Slide |
| Entrez Gene | 54874 |
|---|---|
| Protein Accession | NP_001020119 |
Application
Immunofluorescence of TOCA-1 in Human Brain cells with TOCA-1 antibody at 20 μg/mL.| Positive Control | 1) Cat. No. 1303 - Human Brain Tissue Lysate |
|---|---|
| Application Notes | TOCA-1 antibody can be used for detection of TOCA-1 by Western blot at 0.5 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | TOCA-1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | TOCA-1 Antibody is supplied in PBS containing 0.02% sodium azide. |
