Application
Western blot analysis of TOLLIP in rat brain cell lysate with TOLLIP antibody at (A) 1 and (B) 2 μg/mL.Application
Immunofluorescence of TOLLIP in human brain tissue with TOLLIP antibody at 20 μg/ml.Application
Immunohistochemistry of TOLLIP in human brain tissue with TOLLIP antibody at 5 μg/ml.Application
Immunocytochemistry of TOLLIP in THP-1 cells with TOLLIP antibody at 2 μg/mL.| Product Name | TOLLIP Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | TOLLIP Antibody: IL-1RAcPIP, Toll-interacting protein |
| Product description | TOLLIP Antibody: Toll-like receptors (TLRs) are evolutionarily conserved pattern-recognition molecules resembling the toll proteins that mediate antimicrobial responses in Drosophila. These proteins recognize different microbial products during infection and serve as an important link between the innate and adaptive immune responses. The TLRs act through adaptor molecules to activate various kinases and transcription factors so the organism can respond to potential infection. These adaptor molecules include TOLLIP, MyD88, and TRIF. TOLLIP associates directly with TLR2 and TLR 4, acting as an inhibitor to TLR activation. This negative regulation of TLR signaling may serve to limit the production of proinflammatory mediators during infection and inflammation.1) Takeda K, Kaisho T, and Akira S. Toll-like receptors. Annu. Rev. Immunol.2003; 21:335-76. |
| Immunogen | TOLLIP antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of human TOLLIP. The immunogen is located within the last 50 amino acids of TOLLIP. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCIFIHC-PWB |
| TOLLIP antibody can be used for the detection of TOLLIP by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL and immunocytochemistry starting at 2 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TOLLIP |
|---|---|
| Alternative Names | TOLLIP Antibody: IL-1RAcPIP Toll-interacting protein |
| Sequence Similarities | 3) Cat. No. 17-008 - THP-1 Cell Slide |
Application
Western blot analysis of TOLLIP in rat brain cell lysate with TOLLIP antibody at (A) 1 and (B) 2 μg/mL.
Application
Immunofluorescence of TOLLIP in human brain tissue with TOLLIP antibody at 20 μg/ml.
Application
Immunohistochemistry of TOLLIP in human brain tissue with TOLLIP antibody at 5 μg/ml.
Application
Immunocytochemistry of TOLLIP in THP-1 cells with TOLLIP antibody at 2 μg/mL.| Positive Control | 1) Cat. No. 1463 - Rat Brain Tissue Lysate |
|---|---|
| Application Notes | TOLLIP antibody can be used for the detection of TOLLIP by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL and immunocytochemistry starting at 2 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | TOLLIP antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | TOLLIP Antibody is supplied in PBS containing 0.02% sodium azide. |
