Application
Western blot analysis of TOM70 in rat brain tissue lysate with TOM70 antibody at (A) 1 and (B) 2 μg/mL.Application
Immunofluorescence of TOM70 in Mouse Brain cells with TOM70 antibody at 20 μg/mL.Application
Immunohistochemistry of TOM70 in mouse brain tissue with TOM70 antibody at 2.5 μg/mL.| Product Name | TOM70 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | TOM70 Antibody: KIAA0719, TOM70, Mitochondrial import receptor subunit TOM70, Mitochondrial precursor proteins import receptor |
| Product description | TOM70 Antibody: The translocase of outer mitochondrial membrane (TOM) complex is a multisubunit complex involved in the recognition, unfolding, and translocation of preproteins into the mitochondria. TOM70, an important member of the TOM complex, contains a tetratricopeptide repeat domain similar to those found in cytosolic chaperones such as Hsp90 and Hsc70 and provides a docking site for these proteins. This interaction is thought to be a critical first step in the TOM70-dependent mitochondrial import, followed by contact between the preprotein and TOM70. After targeting to TOM70, preproteins are translocated through the outer membrane via the TOM40 import pore complex. The precise mechanism of how preproteins progress from TOM70 to TOM40 to full translocation is still unclear. At least two isoforms of TOM70 are known to exist.1) Kutik S, Guiard B, Meyer HE, et al. Cooperation of translocase complexes in mitochondrial protein import. J. Cell Biol.2007; 179:585-91. |
| Immunogen | TOM70 antibody was raised against a 15 amino acid synthetic peptide near the center of human TOM70. The immunogen is located within amino acids 220 - 270 of TOM70. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| TOM70 antibody can be used for detection of TOM70 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TOMM70A |
|---|---|
| Alternative Names | TOM70 Antibody: KIAA0719 TOM70 Mitochondrial import receptor subunit TOM70 Mitochondrial precursor proteins import receptor |
Application
Western blot analysis of TOM70 in rat brain tissue lysate with TOM70 antibody at (A) 1 and (B) 2 μg/mL.
Application
Immunofluorescence of TOM70 in Mouse Brain cells with TOM70 antibody at 20 μg/mL.
Application
Immunohistochemistry of TOM70 in mouse brain tissue with TOM70 antibody at 2.5 μg/mL.| Positive Control | 1) Cat. No. 1463 - Rat Brain Tissue Lysate |
|---|---|
| Application Notes | TOM70 antibody can be used for detection of TOM70 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | TOM70 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | TOM70 Antibody is supplied in PBS containing 0.02% sodium azide. |
