Application
Western blot analysis of extracts from HepG2 cells treated with Ca2+ using TOP2A (Phospho-Thr1343) Antibody. The lane on the right is treated with the antigen-specific peptide.| Product Name | TOP2A (phospho Thr1343) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | TP2A, TOP2, TOP2A, P11388-1 |
| Product description | This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This nuclear enzyme is involved in processes such as chromosome condensation, chromatid separation, and the relief of torsional stress that occurs during DNA transcription and replication. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another, thus altering the topology of DNA. Two forms of this enzyme exist as likely products of a gene duplication event.1) Tsai-Pflugfelder M., Proc. Natl. Acad. Sci. U.S.A. 85:7177-7181(1988). |
| Immunogen | TOP2A (phospho Thr1343) antibody was raised against a peptide sequence around phosphorylation site of threonine1343 (E-K-T (p)-D-D) derived from Human TOP2A. |
| Modification | p-Thr1343 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| Western Blot: 1:500~1:1000: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TOP2A |
|---|---|
| Alternative Names | TP2A TOP2 TOP2A P11388-1 |
| Molecular Weight(MW) | 190 kDa |
| Tissue Specificity | The antibody detects endogenous levels of TOP2A only when phosphorylated at threonine 1343. |
| Entrez Gene | 7153 |
|---|---|
| Protein Accession | NP_001058.2 |
Application
Western blot analysis of extracts from HepG2 cells treated with Ca2+ using TOP2A (Phospho-Thr1343) Antibody. The lane on the right is treated with the antigen-specific peptide.| Application Notes | Western Blot: 1:500~1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
