Application
Antibody used in WB on Human HepG2 at 1.25 ug/ml.| Product Name | TOP2A Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | TOP2A, TOP2, TP2A |
| Product description | TOP2A is a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This nuclear enzyme is involved in processes such as chromosome condensation, chromatid separation, and the relief of torsional stress that occurs during DNA transcription and replication. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another, thus altering the topology of DNA. Two forms of this enzyme exist as likely products of a gene duplication event. The gene encoding this form, alpha, is localized to chromsome 17 and the beta gene is localized to chromosome 3. The gene encoding this enzyme functions as the target for several anticancer agents and a variety of mutations in this gene have been associated with the development of drug resistance. Reduced activity of this enzyme may also play a role in ataxia-telangiectasia.1) Yoshida, K., et al., (2006) Mol. Cell. Biol. 26 (9), 3414-3431. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human TOP2A. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| TOP2A antibody can be used for detection of TOP2A by ELISA at 1:62500. TOP2A antibody can be used for detection of TOP2A by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | DogHuman |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | TOP2A |
|---|---|
| Alternative Names | TOP2A TOP2 TP2A |
| Molecular Weight(MW) | 174 kDa |
Application
Antibody used in WB on Human HepG2 at 1.25 ug/ml.| Positive Control | 1) Cat. No. 1211 - HepG2 Cell Lysate |
|---|---|
| Application Notes | TOP2A antibody can be used for detection of TOP2A by ELISA at 1:62500. TOP2A antibody can be used for detection of TOP2A by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store TOP2A antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt |
