Application
Western blot analysis in mouse bladder tissue lysates (35ug/lane).| Product Name | TOP3B Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | DNA topoisomerase 3-beta-1, DNA topoisomerase III beta-1, TOP3B, TOP3B1 |
| Product description | This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This enzyme catalyzes the transient breaking and rejoining of a single strand of DNA which allows the strands to pass through one another, thus relaxing the supercoils and altering the topology of DNA. The enzyme interacts with DNA helicase SGS1 and plays a role in DNA recombination, cellular aging and maintenance of genome stability. Alternative splicing of the C-terminus of this gene results in three transcript variants which have distinct tissue specificity; however, not all variants have been fully described.1) Oliveira-Costa, J.P., et al. Hum. Pathol. 41(11):1624-1630(2010) |
| Immunogen | This TOP3B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 826-854 amino acids from the C-terminal region of human TOP3B. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB starting dilution is: 1:1000: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TOP3B |
|---|---|
| Alternative Names | DNA topoisomerase 3-beta-1 DNA topoisomerase III beta-1 TOP3B TOP3B1 |
| Molecular Weight(MW) | 97 kDa |
Application
Western blot analysis in mouse bladder tissue lysates (35ug/lane).| Application Notes | For WB starting dilution is: 1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
