Application
Western blot analysis of TOPAZ1 in human testis tissue lysate with TOPAZ1 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.Application
Immunofluorescence of TOPAZ1 in rat testis tissue with TOPAZ1 antibody at 20 μg/mL.Application
Immunohistochemistry of TOPAZ1 in rat testis tissue with TOPAZ1 antibody at 5 μg/mL.| Product Name | TOPAZ1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | TOPAZ1 Antibody: C3orf77, C3orf77, Testis- and ovary-specific PAZ domain-containing protein 1 |
| Product description | The testis and ovary specific PAZ domain containing 1 (TOPAZ1) protein is a highly conserved protein that is highly expressed in the gonads during germ cell meiosis, suggesting that it may play a role in germ cell development (1). It contains a PAZ domain and a zinc finger domain and is exclusively expressed in adult testis and fetal ovary.1) Baillet A, Le Bouffant R, Volff JN, et al. TOPAZ1, a novel germ cell-specific expressed gene conserved during evolution across vertebrates. PLoS One 2011; 6:e26950. |
| Immunogen | TOPAZ1 antibody was raised against a 19 amino acid peptide near the carboxy terminus of human TOPAZ1. The immunogen is located within amino acids 1330 - 1380 of TOPAZ1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| TOPAZ1 antibody can be used for detection of TOPAZ1 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry at 5 μg/mL. For Immunoflorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TOPAZ1 |
|---|---|
| Alternative Names | TOPAZ1 Antibody: C3orf77 C3orf77 Testis- and ovary-specific PAZ domain-containing protein 1 |
| Molecular Weight(MW) | Predicted: 65, 186 kDa Observed: 60, 240 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine: (88%), Sheep: (88%) |
| Tissue Specificity | TOPAZ1 antibody is human, mouse and rat reactive. At least three isoforms of TOPAZ1 are known to exist. |
| Entrez Gene | 375337 |
|---|---|
| Protein Accession | NP_001138502 |
Application
Western blot analysis of TOPAZ1 in human testis tissue lysate with TOPAZ1 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.
Application
Immunofluorescence of TOPAZ1 in rat testis tissue with TOPAZ1 antibody at 20 μg/mL.
Application
Immunohistochemistry of TOPAZ1 in rat testis tissue with TOPAZ1 antibody at 5 μg/mL.| Positive Control | 1) Cat. No. 1313 - Human Testis Tissue Lysate |
|---|---|
| Application Notes | TOPAZ1 antibody can be used for detection of TOPAZ1 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry at 5 μg/mL. For Immunoflorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | TOPAZ1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | TOPAZ1 Antibody is supplied in PBS containing 0.02% sodium azide. |
