Application
Western blot analysis of TOSO in K562 cell lysate with TOSO antibody at (A) 1 and (B) 2 μg/mL.| Product Name | Toso Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Toso Antibody: FCMR, TOSO, Fas apoptotic inhibitory molecule 3, Regulator of Fas-induced apoptosis Toso |
| Product description | Toso Antibody: Apoptosis is an important process by which normal tissue homeostasis and function are maintained. One of the major signals that regulate this process is mediated by the activation of the Fas receptor by its ligand. This leads to the formation of a Fas-associated death domain (FADD)- containing death-inducing signaling complex and the activation of caspase-8, which in turn activates downstream effector caspases, such as caspase-3 and -7. Recent experiments have shown that overexpression of Toso, a novel regulator of Fas-induced apoptosis in lymphoid cells, in Jurkat cells as well as transgenic mice render these cells resistant to Fas-induced apoptosis but not to TRAIL-induced apoptosis. Furthermore, Toso was found to associate with FADD, suggesting that Toso functions by disrupting the formation of the death-inducing signaling complex.1) Curtin JF and Cotter TG. Live and let die: regulatory mechanisms in Fas-mediated apoptosis. Cell Signal. 2003; 15:983-92. |
| Immunogen | Toso antibody was raised against a 13 amino acid synthetic peptide from near the carboxy terminus of human Toso. The immunogen is located within the first 50 amino acids of Toso. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| Toso antibody can be used for detection of Toso by Western blot at 1 - 2 μg/mL.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | FAIM3 |
|---|---|
| Alternative Names | Toso Antibody: FCMR TOSO Fas apoptotic inhibitory molecule 3 Regulator of Fas-induced apoptosis Toso |
| Molecular Weight(MW) | Predicted: 43 kDa Observed: 41 kDa |
Application
Western blot analysis of TOSO in K562 cell lysate with TOSO antibody at (A) 1 and (B) 2 μg/mL.| Positive Control | 1) Cat. No. 1204 - K562 Cell Lysate |
|---|---|
| Application Notes | Toso antibody can be used for detection of Toso by Western blot at 1 - 2 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Toso antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Toso Antibody is supplied in PBS containing 0.02% sodium azide. |
