Application
Western blot analysis of TOX in human colon tissue lysate with TOX antibody at (A) 1 and (B) 2 μg/mL.Application
Immunofluorescence of TOX in human colon tissue with TOX antibody at 20 μg/mL.| Product Name | TOX Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | TOX Antibody: TOX1, KIAA0808, Thymocyte selection-associated high mobility group box protein TOX, Thymus high mobility group box protein TOX |
| Product description | TOX Antibody: TOX (thymocyte selection-associated high mobility group (HMG) box protein) is a member of the HMG box family of DNA-binding proteins and likely plays a role in the regulation of T-cell development. It is a 526 amino acid nuclear protein and the expression of TOX is upregulated by pre-T cell receptor (pre-TCR) and TCR activation in immature thymocytes. TOX-mediated positive selection is associated with up-regulation of RUNX3 and is calcineurin dependent. TOX-dependent transition to the CD4+CD8 stage is required for development of class II major histocompatibility complex-specific T cells.1) Wilkinson B, Chen JY, Han P, et al. TOX: an HMG box protein implicated in the regulation of thymocyte selection. Nat. Immunol. 2002; 3:272-80. |
| Immunogen | TOX antibody was raised against a 17 amino acid synthetic peptide near the amino terminus of human TOX. The immunogen is located within amino acids 130 - 180 of TOX. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFWB |
| TOX antibody can be used for detection of TOX by Western blot at 1 - 2 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TOX |
|---|---|
| Alternative Names | TOX Antibody: TOX1 KIAA0808 Thymocyte selection-associated high mobility group box protein TOX Thymus high mobility group box protein TOX |
| Molecular Weight(MW) | 58 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Mouse: (76%) |
| Tissue Specificity | No alternatively spliced transcript variants have been observed. |
Application
Western blot analysis of TOX in human colon tissue lysate with TOX antibody at (A) 1 and (B) 2 μg/mL.
Application
Immunofluorescence of TOX in human colon tissue with TOX antibody at 20 μg/mL.| Positive Control | 1) Cat. No. 1320 - Human Colon Tissue Lysate |
|---|---|
| Application Notes | TOX antibody can be used for detection of TOX by Western blot at 1 - 2 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | TOX antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | TOX Antibody is supplied in PBS containing 0.02% sodium azide. |
