WB
Western blot analysis of TOX on different lysates with Rabbit anti-TOX antibody at 1/5,000 dilution. Low expression: Daudi. Lysates/proteins at 15 µg/Lane. Exposure time: 3 minutes; Blocking: 5% NFDM/TBST, 1 hour at room temperature. Primary antibody: TOX Recombinant Rabbit Monoclonal Antibody, 1/5,000 in primary antibody dilution buffer, overnight at 4 °C. Secondary antibody: Goat anti-Rabbit IgG-HRP, 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature.WB
Western blot analysis of TOX on different lysates with Rabbit anti-TOX antibody at 1/2,000 dilution. Lysates/proteins at 30 µg/Lane. Exposure time: 2 minutes; Negative expression of TOX protein in skeletal muscle is consistent with the predicted expression pattern. Blocking: 5% NFDM/TBST, 1 hour at room temperature. Primary antibody: TOX Recombinant Rabbit Monoclonal Antibody, 1/2,000 in primary antibody dilution buffer, overnight at 4 °C. Secondary antibody: Goat anti-Rabbit IgG-HRP, 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature.IHC
Application: Immunohistochemistry (IHC-P). Species: Human. Tissue: Thymus. Sample: Paraffin-embedded section. Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST. Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: TOX Recombinant Rabbit Monoclonal Antibody, 1/1,000, 1 hour at room temperature. Secondary antibody: 20 minutes at room temperature.IHC
pplication: Immunohistochemistry (IHC-P). Species: Mouse. Tissue: Thymus. Sample: Paraffin-embedded section. Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: TOX Recombinant Rabbit Monoclonal Antibody, 1/1,000, 1 hour at room temperature. Secondary antibody: 20 minutes at room temperature.IHC
Application: Immunohistochemistry (IHC-P). Species: Rat. Tissue: Thymus. Sample: Paraffin-embedded section. Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST. Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: TOX Recombinant Rabbit Monoclonal Antibody, 1/1,000, 1 hour at room temperature. Secondary antibody: 20 minutes at room temperature.ICC/IF
Application: Immunocytochemistry (IF-cell). Species: Human. Sample: Raji (Human Burkitt's lymphoma cell)/ Daudi (Human Burkitt's lymphoma cell). Fixation: 4% Paraformaldehyde, 15 minutes at room temperature. Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature. Antibody dilution buffer: 1% BSA in PBST. Primary antibody: TOX Recombinant Rabbit Monoclonal Antibody, 1/50, overnight at 4°C. Secondary antibody: Goat Anti-Rabbit IgG (488), 45 minutes at room temperature. Counterstain: Beta tubulin (Red), 1/100, overnight at 4℃. The nuclear counterstain was DAPI (Blue). Low expression: Daudi.| Product Name | TOX Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:2000-1:5000 IHC:1:1000 ICC/IF:1:50 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | TOX |
|---|---|
| Gene Synonyms | TOX1 |
| Gene Full Name | thymocyte selection associated high mobility group box |
| Gene Summary | The protein encoded by this gene contains a HMG box DNA binding domain. HMG boxes are found in many eukaryotic proteins involved in chromatin assembly, transcription and replication. This protein may function to regulate T-cell development.[provided by RefSeq, Apr 2009] |
| Alternative Names | Thymocyte selection-associated high mobility group box protein TOX Thymus high mobility group box protein TOX TOX KIAA0808 |
| Molecular Weight(MW) | 57 kDa(Observed band size: 60/75 kDa) |
| Cellular Localization | Nucleus. |
| SwissProt ID | O94900 |
|---|
WB
Western blot analysis of TOX on different lysates with Rabbit anti-TOX antibody at 1/5,000 dilution. Low expression: Daudi. Lysates/proteins at 15 µg/Lane. Exposure time: 3 minutes; Blocking: 5% NFDM/TBST, 1 hour at room temperature. Primary antibody: TOX Recombinant Rabbit Monoclonal Antibody, 1/5,000 in primary antibody dilution buffer, overnight at 4 °C. Secondary antibody: Goat anti-Rabbit IgG-HRP, 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature.
WB
Western blot analysis of TOX on different lysates with Rabbit anti-TOX antibody at 1/2,000 dilution. Lysates/proteins at 30 µg/Lane. Exposure time: 2 minutes; Negative expression of TOX protein in skeletal muscle is consistent with the predicted expression pattern. Blocking: 5% NFDM/TBST, 1 hour at room temperature. Primary antibody: TOX Recombinant Rabbit Monoclonal Antibody, 1/2,000 in primary antibody dilution buffer, overnight at 4 °C. Secondary antibody: Goat anti-Rabbit IgG-HRP, 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature.
IHC
Application: Immunohistochemistry (IHC-P). Species: Human. Tissue: Thymus. Sample: Paraffin-embedded section. Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST. Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: TOX Recombinant Rabbit Monoclonal Antibody, 1/1,000, 1 hour at room temperature. Secondary antibody: 20 minutes at room temperature.
IHC
pplication: Immunohistochemistry (IHC-P). Species: Mouse. Tissue: Thymus. Sample: Paraffin-embedded section. Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: TOX Recombinant Rabbit Monoclonal Antibody, 1/1,000, 1 hour at room temperature. Secondary antibody: 20 minutes at room temperature.
IHC
Application: Immunohistochemistry (IHC-P). Species: Rat. Tissue: Thymus. Sample: Paraffin-embedded section. Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST. Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: TOX Recombinant Rabbit Monoclonal Antibody, 1/1,000, 1 hour at room temperature. Secondary antibody: 20 minutes at room temperature.
ICC/IF
Application: Immunocytochemistry (IF-cell). Species: Human. Sample: Raji (Human Burkitt's lymphoma cell)/ Daudi (Human Burkitt's lymphoma cell). Fixation: 4% Paraformaldehyde, 15 minutes at room temperature. Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature. Antibody dilution buffer: 1% BSA in PBST. Primary antibody: TOX Recombinant Rabbit Monoclonal Antibody, 1/50, overnight at 4°C. Secondary antibody: Goat Anti-Rabbit IgG (488), 45 minutes at room temperature. Counterstain: Beta tubulin (Red), 1/100, overnight at 4℃. The nuclear counterstain was DAPI (Blue). Low expression: Daudi.| Application Notes | WB:1:2000-1:5000 IHC:1:1000 ICC/IF:1:50 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage Buffer | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
