Application
Immunohistochemistry staining of TP53 in brain cortex tissue using TP53 monoclonal Antibody.| Product Name | TP53 Antibody, Monoclonal |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | TP53, BCC7, Cellular tumor antigen p53, p53, TRP53, p53 tumor suppressor, Phosphoprotein p53, Antigen NY-CO-13, Tumor protein p53, Tumor suppressor p53, LFS1 |
| Product description | The p53 gene like the Rb gene, is a tumor suppressor gene, i.e., its activity stops the formation of tumors. If a person inherits only one functional copy of the p53 gene from their parents, they are pre-disposed to cancer and usually develop several independent tumors in a variety of tissues in early adulthood. This condition is rare, and is known as Li-Fraumeni syndrome. However, mutations in p53 are found in most tumor types, and so contribute to the complex network of molecular events leading to tumor formation. In the cell, p53 protein binds DNA, which in turn stimulates another gene to produce a protein called p21 that interacts with a cell division-stimulating protein (cdk2). When p21 is complexed with cdk2 the cell cannot pass through to the next stage of cell division. Mutant p53 can no longer bind DNA in an effective way, and as a consequence the p21 protein is not made available to act as the 'stop signal' for cell division. Thus cells divide uncontrollably, and form tumors. p53 localizes in the nucleus, but is detectable at the plasma membrane during mitosis and when certain mutations modulate cytoplasmic/nuclear distribution. The amount of information that exists on all aspects of p53 normal function and mutant expression in human cancers is now vast, reflecting its key role in the pathogenesis of human cancers. It is clear that p53 is just one component of a network of events that culminate in tumor formation. |
| Immunogen | TP53 monoclonal antibody was raised against amino acids 1 - 534 of TP53 (Human) using recombinant human p53 protein. Hybridoma: Produced by the fusion between BALB/c mouse splenocytes and mouse myeloma P3-X63/AG8.653 cells using conventional hybridoma tech |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a,k |
| Host Species | Mouse |
| Tested Applications | ELISAIHCIPWB |
| TP53 antibody can be used in ELISA starting at 1:1000, and immunohistochemistry starting at 10 μg/mL.: |
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| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | TP53 |
|---|---|
| Alternative Names | TP53 BCC7 Cellular tumor antigen p53 p53 TRP53 p53 tumor suppressor Phosphoprotein p53 Antigen NY-CO-13 Tumor protein p53 Tumor suppressor p53 LFS1 |
Application
Immunohistochemistry staining of TP53 in brain cortex tissue using TP53 monoclonal Antibody.| Application Notes | TP53 antibody can be used in ELISA starting at 1:1000, and immunohistochemistry starting at 10 μg/mL.: |
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| Form | Liquid |
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| Storage Instructions | Store TP53 antibody at 4 ˚C or -20 ˚C. As with all antibodies avoid freeze/thaw cycles. |
| Storage Buffer | 0.02 M potassium phosphate, 0.5 M sodium chloride, pH 7.2, 0.01% sodium azide. |
