TPI1 belongs to the triosephosphate isomerase family. Defects in TPI1 are the cause of triosephosphate isomerase deficiency (TPI deficiency) . TPI deficiency is an autosomal recessive disorder. It is the most severe clinical disorder of glycolysis. It is associated with neonatal jaundice, chronic hemolytic anemia, progressive neuromuscular dysfunction, cardiomyopathy and increased susceptibility to infection.1) Ralser, M., (er) BMC Genet. 9, 38 (2008).
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human TPI1.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAWB
TPI1 antibody can be used for detection of TPI1 by ELISA at 1:1562500. TPI1 antibody can be used for detection of TPI1 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Antibody used in WB on Human HepG2 at 0.2-1 ug/ml.
Application
Antibody used in WB on Human, Drosophila.
Positive Control
1) Cat. No. 1211 - HepG2 Cell Lysate
Application Notes
TPI1 antibody can be used for detection of TPI1 by ELISA at 1:1562500. TPI1 antibody can be used for detection of TPI1 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store TPI1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.