WB
Western blot analysis of various lysates using TPI1 Rabbit pAb at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.IHC
Immunohistochemistry analysis of paraffin embedded human lung cancer using TPI1 Rabbit pAb at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.ICC/IF
Immunofluorescence analysis of U-2 OS cells using TPI1 Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3 Goat Anti Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.| Product Name | TPI1 Rabbit pAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 1-249 of human TPI1 (NP_000356.1). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:500-1:2000 IHC:1:100-1:200 ICC:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TPI1 |
|---|---|
| Gene Synonyms | TIM TPI TPID HEL-S-49 |
| Gene Full Name | triosephosphate isomerase 1 |
| Gene Summary | This gene encodes an enzyme, consisting of two identical proteins, which catalyzes the isomerization of glyceraldehydes 3-phosphate (G3P) and dihydroxy-acetone phosphate (DHAP) in glycolysis and gluconeogenesis. Mutations in this gene are associated with triosephosphate isomerase deficiency. Pseudogenes have been identified on chromosomes 1, 4, 6 and 7. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2009] |
| Alternative Names | TIM TPI TPID HEL-S-49 TPI1 |
| Molecular Weight(MW) | 27kDa( Observed MW 30kDa) |
| Cellular Localization | cytosol,extracellular exosome,extracellular space,nucleus |
WB
Western blot analysis of various lysates using TPI1 Rabbit pAb at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
IHC
Immunohistochemistry analysis of paraffin embedded human lung cancer using TPI1 Rabbit pAb at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ICC/IF
Immunofluorescence analysis of U-2 OS cells using TPI1 Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3 Goat Anti Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.| Application Notes | WB:1:500-1:2000 IHC:1:100-1:200 ICC:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3. |
