Application
Western blot analysis in U251,A549,Jurkat cell line lysates (35ug/lane).Application
Flow cytometric analysis of U251 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | TPM4 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Tropomyosin alpha-4 chain, TM30p1, Tropomyosin-4, TPM4 |
| Product description | This gene encodes a member of the tropomyosin family of actin-binding proteins involved in the contractile system of striated and smooth muscles and the cytoskeleton of non-muscle cells. Tropomyosins are dimers of coiled-coil proteins that polymerize end-to-end along the major groove in most actin filaments. They provide stability to the filaments and regulate access of other actin-binding proteins. In muscle cells, they regulate muscle contraction by controlling the binding of myosin heads to the actin filament. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq].1) Martins-de-Souza, D., et al.Psychiatr Res 44(14):989-991(2010) |
| Immunogen | This TPM4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 26-54 amino acids from the N-terminal region of human TPM4. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSWB |
| For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TPM4 |
|---|---|
| Alternative Names | Tropomyosin alpha-4 chain TM30p1 Tropomyosin-4 TPM4 |
| Molecular Weight(MW) | 29 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Pig |
Application
Western blot analysis in U251,A549,Jurkat cell line lysates (35ug/lane).
Application
Flow cytometric analysis of U251 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
