Application
Western blot analysis in MDA-MB231 cell line lysates (35ug/lane).Application
TPSD1 antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.| Product Name | TPSD1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Tryptase delta, Delta-tryptase, HmMCP-3-like tryptase III, Mast cell mMCP-7-like, Tryptase-3, TPSD1 |
| Product description | Tryptases comprise a family of trypsin-like serine proteases, the peptidase family S1. Tryptases are enzymatically active only as heparin-stabilized tetramers, and they are resistant to all known endogenous proteinase inhibitors. Several tryptase genes are clustered on chromosome 16p13.3. These genes are characterized by several distinct features. They have a highly conserved 3' UTR and contain tandem repeat sequences at the 5' flank and 3' UTR which are thought to play a role in regulation of the mRNA stability. Although this gene may be an exception, most of the tryptase genes have an intron immediately upstream of the initiator Met codon, which separates the site of transcription initiation from protein coding sequence. This feature is characteristic of tryptases but is unusual in other genes. Tryptases have been implicated as mediators in the pathogenesis of asthma and other allergic and inflammatory disorders. TPSD1 was once considered to be a pseudogene, although it is now believed to be a functional gene that encodes a protein.1) Jackson, N.E., et al. J. Biol. Chem. 283(49):34178-34187(2008) |
| Immunogen | This TPSD1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 164-193 amino acids from the C-terminal region of human TPSD1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TPSD1 |
|---|---|
| Alternative Names | Tryptase delta Delta-tryptase HmMCP-3-like tryptase III Mast cell mMCP-7-like Tryptase-3 TPSD1 |
| Molecular Weight(MW) | 27 kDa |
Application
Western blot analysis in MDA-MB231 cell line lysates (35ug/lane).
Application
TPSD1 antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
