TPX2 binding decreases the size and accessibility of a hydrophobic pocket, adjacent to the ATP site, to inhibitors.1) Szczepanowski, M., (2007) Biochem. Biophys. Res. Commun. 362 (2), 485-490.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human TPX2.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAWB
TPX2 antibody can be used for detection of TPX2 by ELISA at 1:62500. TPX2 antibody can be used for detection of TPX2 by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Antibody used in WB on Human 721_B at 0.2-1 ug/ml.
Positive Control
1) 721_B Cell Lysate
Application Notes
TPX2 antibody can be used for detection of TPX2 by ELISA at 1:62500. TPX2 antibody can be used for detection of TPX2 by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store TPX2 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.