Application
Western blot analysis in A549 cell line lysates (35ug/lane).| Product Name | TPX2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Targeting protein for Xklp2, Differentially expressed in cancerous and non-cancerous lung cells 2, DIL-2, Hepatocellular carcinoma-associated antigen 519, Hepatocellular carcinoma-associated antigen 90, Protein fls353, Restricted expression proliferation-a |
| Product description | TPX2 is a spindle assembly factor. Required for normal assembly of mitotic spindles. Required for normal assembly of microtubules during apoptosis. Required for chromatin and/or kinetochore dependent microtubule nucleation. Mediates AURKA localization to spindle microtubules. Activates AURKA by promoting its autophosphorylation at 'Thr-288' and protects this residue against dephosphorylation.1) Olson, J.E., et al. Breast Cancer Res. Treat. (2010) In press : |
| Immunogen | This TPX2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 419-448 amino acids from the Central region of human TPX2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB starting dilution is: 1:1000: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TPX2 |
|---|---|
| Alternative Names | Targeting protein for Xklp2 Differentially expressed in cancerous and non-cancerous lung cells 2 DIL-2 Hepatocellular carcinoma-associated antigen 519 Hepatocellular carcinoma-associated antigen 90 Protein fls353 Restricted expression proliferation-a |
| Molecular Weight(MW) | 86 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine |
Application
Western blot analysis in A549 cell line lysates (35ug/lane).| Application Notes | For WB starting dilution is: 1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
