Application
Western blot analysis of TRAF6 in PC-3 cell lysates with TRAF6 antibody at 1 μg/mL in the presence (A) or absence (B) of 1 μg blocking peptide.Application
Immunofluorescence of TRAF6 in K562 cells with TRAF6 antibody at 20 μg/mL.Application
Immunocytochemistry of TRAF6 in K562 cells with TRAF6 antibody at 0.5 μg/mL.| Product Name | TRAF6 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | TRAF6 Antibody: RNF85, MGC:3310, RNF85, E3 ubiquitin-protein ligase TRAF6 |
| Product description | TRAF6 Antibody: Signals from the IL-1 receptor (IL-1R)/Toll-like receptor (TLR) and TNF receptor (TNFR) superfamilies are critical for regulating the function of antigen-presenting cells. Signals transduced by these molecules lead to increased expression and activation of transcription factors such as NF-κB. TNF receptor-associated factor 6 (TRAF6) is unique in that it is a signaling adapter molecule common to both families. TRAF6 is important in cytokine production, dendritic cell (DC) maturation, and the T cell stimulatory capacity of DCs in response to TLR and CD40 ligands. It can be activated in the IL-1R/TLR signaling pathway by IL-1 receptor-associated kinase 1 (IRAK-1) or by other TLR adaptor molecules such as TRIF. Also, it has been shown that TRAF6 can interact directly with TNFR family members CD40 and RANK.1) Takeda K, Kaisho T, and Akira S. Toll-like receptors. Annu. Rev. Immunol. 2003; 21:335-76. |
| Immunogen | TRAF6 antibody was raised against a peptide corresponding to 14 amino acids near the C-terminus of human TRAF6. The immunogen is located within the last 50 amino acids of TRAF6. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCIFWB |
| TRAF6 antibody can be used for detection of TRAF6 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TRAF6 |
|---|---|
| Alternative Names | TRAF6 Antibody: RNF85 MGC:3310 RNF85 E3 ubiquitin-protein ligase TRAF6 |
| Sequence Similarities | 3) Cat. No. 17-004 - K-562 Cell Slide |
Application
Western blot analysis of TRAF6 in PC-3 cell lysates with TRAF6 antibody at 1 μg/mL in the presence (A) or absence (B) of 1 μg blocking peptide.
Application
Immunofluorescence of TRAF6 in K562 cells with TRAF6 antibody at 20 μg/mL.
Application
Immunocytochemistry of TRAF6 in K562 cells with TRAF6 antibody at 0.5 μg/mL.| Positive Control | 1) Cat. No. 1216 - PC-3 Cell Lysate |
|---|---|
| Application Notes | TRAF6 antibody can be used for detection of TRAF6 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | TRAF6 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | TRAF6 Antibody is supplied in PBS containing 0.02% sodium azide. |
