Application
Fluorescent image of MCF-7 cell stained with TREF1 Antibody .MCF-7 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with TREF1 primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7uniApplication
Western blot analysis of TREF1 Antibody in K562 cell line lysates (35ug/lane)Application
Flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | TREF1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Transcriptional-regulating factor 1, Breast cancer anti-estrogen resistance 2, Transcriptional-regulating protein 132, Zinc finger protein rapa, Zinc finger transcription factor TReP-132, TRERF1, BCAR2, RAPA, TREP132 |
| Product description | TREF1 encodes a zinc-finger transcriptional regulating protein which interacts with CBP/p300 to regulate the human gene CYP11A1.1) Gizard,F. Mol. Cell. Biol. 26 (20), 7632-7644 (2006) |
| Immunogen | This TREF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1136-1164 amino acids from the C-terminal region of human TREF1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIFWB |
| For IF starting dilution is: 1:10~50 For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TRERF1 |
|---|---|
| Alternative Names | Transcriptional-regulating factor 1 Breast cancer anti-estrogen resistance 2 Transcriptional-regulating protein 132 Zinc finger protein rapa Zinc finger transcription factor TReP-132 TRERF1 BCAR2 RAPA TREP132 |
| Molecular Weight(MW) | 132 kDa |
Application
Fluorescent image of MCF-7 cell stained with TREF1 Antibody .MCF-7 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with TREF1 primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7uni
Application
Western blot analysis of TREF1 Antibody in K562 cell line lysates (35ug/lane)
Application
Flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | For IF starting dilution is: 1:10~50 For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
