Application
Western blot analysis of TREX2 in rat liver tissue lysate with TREX2 antibody at 2.5 μg/mL.Application
Immunofluorescence of TREX2 in Human Liver cells with TREX2 antibody at 20 μg/mL.Application
Immunohistochemistry of TREX2 in human liver tissue with TREX2 antibody at 2.5 μg/mL.| Product Name | TREX2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | TREX2 Antibody: Three prime repair exonuclease 2 |
| Product description | TREX2 Antibody: TREX2 is one of two major human 3' to 5' exonucleases which are required for checkpoint signaling after DNA damage. While the related protein TREX1 is required for ATR expression and mutations in this gene result in Aicardi-Goutieres syndrome, chilblain lupus, and Cree encephalitis, less is known about TREX2. Like TREX1, TREX2 is ubiquitously expressed in all tissues examined. X-ray crystallography studies of TREX2-single strand DNA complexes revealed that TREX2 binds DNA as a dimer and may act to displace the second DNA strand, suggesting that TREX2 unzips and denatures double stranded DNA and feed the substrate DNA strand into the TREX2 active site. At least two isoforms of TREX2 are known to exist.1) Mazur DJ and Perrino FW. Identification and expression of the TREX1 and TREX2 cDNA sequences encoding mammalian 3→5 exonucleases. J. Biol. Chem.1999; 274:19655-60. |
| Immunogen | TREX2 antibody was raised against a 15 amino acid synthetic peptide near the carboxy terminus of human TREX2. The immunogen is located within the last 50 amino acids of TREX2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| TREX2 antibody can be used for detection of TREX2 by Western blot at 2.5 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TREX2 |
|---|---|
| Alternative Names | TREX2 Antibody: Three prime repair exonuclease 2 |
| Sequence Similarities | 2) Cat. No. 10-201 - Human Liver Tissue Slide |
| Tissue Specificity | This TREX2 antibody will not cross-react with the related protein TREX1. |
Application
Western blot analysis of TREX2 in rat liver tissue lysate with TREX2 antibody at 2.5 μg/mL.
Application
Immunofluorescence of TREX2 in Human Liver cells with TREX2 antibody at 20 μg/mL.
Application
Immunohistochemistry of TREX2 in human liver tissue with TREX2 antibody at 2.5 μg/mL.| Positive Control | 1) Cat. No. 1464 - Rat Liver Tissue Lysate |
|---|---|
| Application Notes | TREX2 antibody can be used for detection of TREX2 by Western blot at 2.5 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | TREX2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | TREX2 Antibody is supplied in PBS containing 0.02% sodium azide. |
