Application
Western blot analysis in MDA-MB435 cell line lysates (15ug/lane).Application
Flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | TRHDE Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Thyrotropin-releasing hormone-degrading ectoenzyme, TRH-DE, TRH-degrading ectoenzyme, Pyroglutamyl-peptidase II, PAP-II, TRH-specific aminopeptidase, Thyroliberinase, TRHDE |
| Product description | This gene encodes a member of the peptidase M1 family. TRHDE is an extracellular peptidase that specifically cleaves and inactivates the neuropeptide thyrotropin-releasing hormone.1) Cirulli, E.T., et al. Eur. J. Hum. Genet. (2010) In press : |
| Immunogen | This TRHDE antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 951-980 amino acids from the C-terminal region of human TRHDE. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSWB |
| For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TRHDE |
|---|---|
| Alternative Names | Thyrotropin-releasing hormone-degrading ectoenzyme TRH-DE TRH-degrading ectoenzyme Pyroglutamyl-peptidase II PAP-II TRH-specific aminopeptidase Thyroliberinase TRHDE |
| Molecular Weight(MW) | 117 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Mouse |
Application
Western blot analysis in MDA-MB435 cell line lysates (15ug/lane).
Application
Flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
