Application
Western blot analysis in K562 cell line lysates (35ug/lane).| Product Name | TRIM27 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Zinc finger protein RFP, 632-, RING finger protein 76, Ret finger protein, Tripartite motif-containing protein 27, TRIM27, RFP, RNF76 |
| Product description | This gene encodes a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This protein localizes to the nuclear matrix. It interacts with the enhancer of polycomb protein and represses gene transcription. It is also thought to be involved in the differentiation of male germ cells. Fusion of the N-terminus of this protein with the truncated C-terminus of the RET gene product has been shown to result in production of the ret transforming protein.1) Johnson, A.D., et al. Nat. Genet. 42(7):608-613(2010) |
| Immunogen | This TRIM27 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 453-482 amino acids from the C-terminal region of human TRIM27. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB starting dilution is: 1:1000: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TRIM27 |
|---|---|
| Alternative Names | Zinc finger protein RFP 632- RING finger protein 76 Ret finger protein Tripartite motif-containing protein 27 TRIM27 RFP RNF76 |
| Molecular Weight(MW) | 58 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Mouse |
Application
Western blot analysis in K562 cell line lysates (35ug/lane).| Application Notes | For WB starting dilution is: 1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
