WB
Various lysates were subjected to SDS PAGE followed by western blot with TRIM27 antibody at dilution of 1:3000 incubated at room temperature for 1.5 hours.IHC
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using TRIM27 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).IP
IP result of anti-TRIM27 (IP:4ug; Detection:1:1000) with mouse testis tissue lysate 4000ug.| Product Name | TRIM27 Polyclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant human TRIM27 protein |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCIPWB |
| WB:1:1000-1:6000 IHC:1:50-1:500 IP:0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.65mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TRIM27 |
|---|---|
| Gene Synonyms | RFP RNF76 |
| Gene Full Name | tripartite motif containing 27 |
| Gene Summary | This gene encodes a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This protein localizes to the nuclear matrix. It interacts with the enhancer of polycomb protein and represses gene transcription. It is also thought to be involved in the differentiation of male germ cells. Fusion of the N-terminus of this protein with the truncated C-terminus of the RET gene product has been shown to result in production of the ret transforming protein. [provided by RefSeq, Jul 2008] |
| Alternative Names | EC:2.3.2.27 RFP RNF76 TRIM Tripartite motif-containing protein 27 |
| Molecular Weight(MW) | 58 kDa, 41 kDa |
| Cellular Localization | Nucleus,Cytoplasm. |
WB
Various lysates were subjected to SDS PAGE followed by western blot with TRIM27 antibody at dilution of 1:3000 incubated at room temperature for 1.5 hours.
IHC
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using TRIM27 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IP
IP result of anti-TRIM27 (IP:4ug; Detection:1:1000) with mouse testis tissue lysate 4000ug.| Application Notes | WB:1:1000-1:6000 IHC:1:50-1:500 IP:0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Stable for one year after shipment. |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
