Application
Western blot analysis of TRIM30 in mouse heart tissue lysate with TRIM30 antibody at (A) 1 and (B) 2 μg/mL.Application
Immunofluorescence of TRIM30 in Human Ovary cells with TRIM30 antibody at 20 μg/mL.Application
Immunohistochemistry of TRIM30 in mouse ovary tissue with TRIM30 antibody at 10 μg/mL.| Product Name | TRIM30 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | TRIM30 Antibody: Rpt1, Rpt-1, Trim30, Rpt1, Tripartite motif-containing protein 30A, Down regulatory protein of interleukin-2 receptor |
| Product description | TRIM30 Antibody: TRIM30 belongs to a family of the tripartite motif (TRIM) proteins involved in the regulation of cell proliferation, differentiation, development, oncogenesis, apoptosis and antiviral responses. The TRIM protein family is an expanding family of RING ('really interesting new gene') proteins, also known as RBCC proteins as they contain an RBCC motif, which comprises a RING domain, one or two B-boxes and a predicted coiled-coil region. Studies have shown that some TRIM family members are critical to innate immunity; TRIM5, TRIM19 and TRIM25, for example, have been shown to restrict viral infection. A recent study shows that TRIM30 functions as a negative modulator of the TLR signaling pathway, by targeting TAB2 and TAB3, and contributes to the inhibition of TLR-mediated NF-κB activation. The importance of TRIM30 in the attenuation or termination of NF-κB activation suggests that targeting of TAB2 and TAB3 by TRIM30alpha may be a mechanism for modulating many types of immune responses.1) Nisole S, Stoye JP, and Saib A. TRIM family proteins: retroviral restriction and antiviral defence. Nat. Rev. Microbiol.2005; 3:799-808. |
| Immunogen | TRIM30 antibody was raised against a 24 amino acid synthetic peptide near the amino terminus of the mouse TRIM30. The immunogen is located within amino acids 110 - 160 of TRIM30. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| TRIM30 antibody can be used for detection of TRIM30 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | MouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | Trim30 |
|---|---|
| Alternative Names | TRIM30 Antibody: Rpt1 Rpt-1 Trim30 Rpt1 Tripartite motif-containing protein 30A Down regulatory protein of interleukin-2 receptor |
| Molecular Weight(MW) | Predicted: 107 kDa Observed: 105 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Human: (80%) |
Application
Western blot analysis of TRIM30 in mouse heart tissue lysate with TRIM30 antibody at (A) 1 and (B) 2 μg/mL.
Application
Immunofluorescence of TRIM30 in Human Ovary cells with TRIM30 antibody at 20 μg/mL.
Application
Immunohistochemistry of TRIM30 in mouse ovary tissue with TRIM30 antibody at 10 μg/mL.| Positive Control | 1) Cat. No. 1401 - Mouse Heart Tissue Lysate |
|---|---|
| Application Notes | TRIM30 antibody can be used for detection of TRIM30 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | TRIM30 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | TRIM30 Antibody is supplied in PBS containing 0.02% sodium azide. |
