Application
Western blot analysis of TRIM33 in human liver tissue lysate with TRIM33 antibody at (A) 1 and (B) 2 μg/mL.Application
Immunofluorescence of TRIM33 in human liver tissue with TRIM33 antibody at 20 μg/ml.Application
Immunohistochemistry of TRIM33 in human liver tissue with TRIM33 antibody at 2.5 μg/ml.| Product Name | TRIM33 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | TRIM33 Antibody: ECTO, PTC7, RFG7, TF1G, TIF1G, TIFGAMMA, TIF1GAMMA, KIAA1113, E3 ubiquitin-protein ligase TRIM33, Ectodermin homolog, Protein Rfg7 |
| Product description | TRIM33 Antibody: TRIM24 (TIF1alpha), TRIM28 (TIF1beta), and TRIM33 (TIF1gamma) are three related cofactors belonging to the tripartite motif superfamily that interact with distinct transcription factors (1-2). The structure of TRIM33 is similar to TRIM24 and TRIM28, exhibiting multiple domains (RING finger, coiled coil, B boxes, PHD/TTC, and bromodomain) (3). TRIM33 functions in cell differentiation and development, playing a role in differentiation of hematopoietic cells (3). It interacts with the Smad2/3 transcription factor in hematopoietic, mesenchymal, and epithelial cell types to mediate different transcriptional effects in response to TGF-beta (4). It acts as an E3 ubiquitin-protein ligase and promotes SMAD4 ubiquitination (5).1) Ransom DG, Bahary N, Niss K, et al. The zebrafish moonshine gene encodes transcriptional intermediary factor 1gamma, an essential regulator of hematopoiesis. PLoS Biol. 2004; 2:E237. |
| Immunogen | TRIM33 antibody was raised against an 18 amino acid peptide near the center of human TRIM33. The immunogen is located within amino acids 580 - 630 of TRIM33. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| TRIM33 antibody can be used for detection of TRIM33 by Western blot at 1 - 2 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TRIM33 |
|---|---|
| Alternative Names | TRIM33 Antibody: ECTO PTC7 RFG7 TF1G TIF1G TIFGAMMA TIF1GAMMA KIAA1113 E3 ubiquitin-protein ligase TRIM33 Ectodermin homolog Protein Rfg7 |
| Molecular Weight(MW) | Predicted: 70, 124 kDa Observed: 70 kDa |
| Sequence Similarities | 2) Cat. No. 10-201 - Human Liver Tissue Slide |
| Tissue Specificity | TRIM33 antibody is human, mouse and rat reactive. At least two isoforms of TRIM33 are known to exist. |
Application
Western blot analysis of TRIM33 in human liver tissue lysate with TRIM33 antibody at (A) 1 and (B) 2 μg/mL.
Application
Immunofluorescence of TRIM33 in human liver tissue with TRIM33 antibody at 20 μg/ml.
Application
Immunohistochemistry of TRIM33 in human liver tissue with TRIM33 antibody at 2.5 μg/ml.| Positive Control | 1) Cat. No. 1304 - Human Liver Tissue Lysate |
|---|---|
| Application Notes | TRIM33 antibody can be used for detection of TRIM33 by Western blot at 1 - 2 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | TRIM33 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | TRIM33 Antibody is supplied in PBS containing 0.02% sodium azide. |
