The protein encoded by the TRIM68 gene contains a RING finger domain, a motif present in a variety of functionally distinct proteins and known to be involved in protein-protein and protein-DNA interactions. TRIM68 is expressed in many cancer cell lines. Its expression in normal tissues, however, was found to be restricted to prostate. This gene was also found to be differentially expressed in androgen-dependent versus androgen-independent prostate cancer cells.1) Chang, G.T., et al., (2001) Eur. J. Cancer 37 (16), 2129-2134.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human TRIM68.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAIHCWB
TRIM68 antibody can be used for detection of TRIM68 by ELISA at 1:62500. TRIM68 antibody can be used for detection of TRIM68 by western blot at 1.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Antibody used in WB on Human DU145 at 0.2-1 ug/ml.
Positive Control
1) Du145 Cell Lysate
Application Notes
TRIM68 antibody can be used for detection of TRIM68 by ELISA at 1:62500. TRIM68 antibody can be used for detection of TRIM68 by western blot at 1.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store TRIM68 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.