Application
Western blot analysis of TRIP12 in rat colon tissue lysate with TRIP12 antibody at 1 μg/ml.Application
Immunofluorescence of TRIP12 in rat colon tissue with TRIP12 antibody at 20 μg/ml.Application
Immunohistochemistry of TRIP12 in rat colon tissue with TRIP12 antibody at 5 μg/ml.| Product Name | TRIP12 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Thyroid hormone receptors (TRs) are transcription factors that regulate the expression of specific genes in a hormone-dependent manner (1). TRIP12 (thyroid hormone receptor interactor 12) is an ATP-dependent E3 ubiquitin ligase involved in the human ubiquitin fusion degradation (UFD) pathway and also modulates the NEDD8 pathway (2,3). TRIP12 contains one WWE domain and a single HECT (E6AP-type E3 ubiquitin-protein ligase) domain suggested to contain a noncovalent ubiquitin-binding site (4). TRIP12 acts as a key regulator of DNA damage response and the ubiquitin ligase activity of TRIP12 is essential for mouse development (5).1) Lee JW, Choi HS, Gyuris J, et al. Two classes of proteins dependent on either the presence or absence of thyroid hormone for interaction with the thyroid hormone receptor. Mol. Endocrinol. 1995; 9:243–54. |
| Immunogen | TRIP12 antibody was raised against a 18 amino acid peptide near the carboxy terminus of human TRIP12. The immunogen is located within amino acids 1930 - 1980 of TRIP12. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| TRIP12 antibody can be used for detection of TRIP12 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TRIP12 |
|---|---|
| Alternative Names | TRIP12 Antibody: ULF TRIP-12 KIAA0045 ULF E3 ubiquitin-protein ligase TRIP12 E3 ubiquitin-protein ligase for Arf |
| Molecular Weight(MW) | Predicted: 219 kDa Observed: 220 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine: (100%) |
| Tissue Specificity | TRIP12 antibody is human, mouse and rat reactive. At least four isoforms are known to exist. |
Application
Western blot analysis of TRIP12 in rat colon tissue lysate with TRIP12 antibody at 1 μg/ml.
Application
Immunofluorescence of TRIP12 in rat colon tissue with TRIP12 antibody at 20 μg/ml.
Application
Immunohistochemistry of TRIP12 in rat colon tissue with TRIP12 antibody at 5 μg/ml.| Positive Control | 1) Cat. No. 1472 - Rat Colon Tissue Lysate |
|---|---|
| Application Notes | TRIP12 antibody can be used for detection of TRIP12 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | TRIP12 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | TRIP12 antibody is supplied in PBS containing 0.02% sodium azide. |
