TRMT11 belongs to the methyltransferase superfamily. It is a catalytic subunit of an S-adenosyl-L-methionine-dependent tRNA methyltransferase complex that mediates the methylation of the guanosine nucleotide at position 10 (m2G10) in tRNAs.1) Rush, J., (2005) Nat. Biotechnol. 23 (1), 94-101.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human TRMT11.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAWB
TRMT11 antibody can be used for detection of TRMT11 by ELISA at 1:12500. TRMT11 antibody can be used for detection of TRMT11 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Antibody used in WB on Human NTERA2 at 0.2-1 ug/ml.
Positive Control
1) NTERA2 Cell Lysate
Application Notes
TRMT11 antibody can be used for detection of TRMT11 by ELISA at 1:12500. TRMT11 antibody can be used for detection of TRMT11 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store TRMT11 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.