TRNT1 belongs to the tRNA nucleotidyltransferase/poly (A) polymerase family. It adds and repairs the conserved 3'-CCA sequence necessary for the attachment of amino acids to the 3' terminus of tRNA molecules, using CTP and ATP as substrates.1) Augustin, M.A., (2003) J. Mol. Biol. 328 (5), 985-994.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human TRNT1.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAIHCWB
TRNT1 antibody can be used for detection of TRNT1 by ELISA at 1:62500. TRNT1 antibody can be used for detection of TRNT1 by western blot at 2.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Antibody used in IHC on Human Kidney at 4.0-8.0 ug/ml.
Positive Control
1) Cat. No. XBL-10123 - Fetal Brain Tissue Lysate
Application Notes
TRNT1 antibody can be used for detection of TRNT1 by ELISA at 1:62500. TRNT1 antibody can be used for detection of TRNT1 by western blot at 2.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store TRNT1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt