Application
Western blot analysis of Trophinin in rat liver tissue lysate with Trophinin antibody at 1 μg/ml.Application
Immunofluorescence of Trophinin in mouse liver tissue with Trophinin antibody at 20 μg/ml.Application
Immunohistochemistry of TROPHININ in mouse liver tissue with TROPHININ antibody at 10 μg/ml.| Product Name | Trophinin Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | TRO, MAGE-d3, MAGED3 |
| Product description | Trophinin, also known as TRO or MAGED3, is an apical cell adhesion molecule that is implicated in the initial attachment during the process of embryo implantation and functions by mediating cell adhesion between trophoblastic and endometrial epithelial cells (1,2). Trophinin is a membrane protein expressed in chorionic villi trophoblasts and in maternal endometrial epithelial cells in an implantation-dependent manner and interacts with Bystin and Tastin, facilitating cell adhesion and embryo implantation (3,4). The induction of Trophinin expression may be a useful method for improving implantation rates and can be a potential diagnostic factor and biomarker for human cancer (4,5).1) Fukuda MN, Sato T, Nakayama J, et al. Trophinin and tastin, a novel cell adhesion molecule complex with potential involvement in embryo implantation. Genes Dev. 1995; 9:1199-210. |
| Immunogen | Trophinin antibody was raised against a 16 amino acid peptide near the amino terminus of human Trophinin. The immunogen is located within amino acids 390 - 440 of Trophinin. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| Trophinin antibody can be used for detection of Trophinin by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry at 10 μg/ml.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TRO |
|---|---|
| Alternative Names | TRO MAGE-d3 MAGED3 |
| Molecular Weight(MW) | Predicted: 78, 157 kDa Observed: 94 kDa |
| Tissue Specificity | Trophinin antibody is human, mouse and rat reactive. Multiple isoforms of Trophinin are known to exist. |
| Entrez Gene | 7216 |
|---|---|
| Protein Accession | NP_001034794 |
Application
Western blot analysis of Trophinin in rat liver tissue lysate with Trophinin antibody at 1 μg/ml.
Application
Immunofluorescence of Trophinin in mouse liver tissue with Trophinin antibody at 20 μg/ml.
Application
Immunohistochemistry of TROPHININ in mouse liver tissue with TROPHININ antibody at 10 μg/ml.| Positive Control | 1) Cat. No. 1464 - Rat Liver Tissue Lysate |
|---|---|
| Application Notes | Trophinin antibody can be used for detection of Trophinin by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry at 10 μg/ml.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Trophinin antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | Trophinin antibody is supplied in PBS containing 0.02% sodium azide. |
Data sheet for OM294160