Application
Western blot analysis of TRPC3 in human brain tissue lysate with TRPC3 antibody at (A) 1 and (B) 2 μg/mL.Application
Immunofluorescence of TRPC3 in Mouse Heart cells with TRPC3 antibody at 20 μg/mL.Application
Immunohistochemistry of TRPC3 in mouse heart tissue with TRPC3 antibody at 2 μg/mL.| Product Name | TRPC3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | TRPC3 Antibody: TRP3, TRP3, Short transient receptor potential channel 3, Transient receptor protein 3, TrpC3 |
| Product description | TRPC3 Antibody: The mammalian transient receptor potential (TRP) superfamily can be divided into three major families including the "canonical TRP" (TRPC) family. The seven members of this family share the activation through PLC-coupled receptors and have been suggested to be components of receptor-regulated cation channels in different cell types. Furthermore, the members of the TRPC3/6/7 subfamily can be activated by diacylglycerol analogs, suggesting a possible mechanism of activation of these channels by PLC-coupled receptors. TRPC3 encodes a Ca2+-permeant channel that is agonist-activated but not store-operated or directly receptor-activated. TRPC3 physically interacts with TRPC6 and TRPC7 and forms functional tetrameric channels.1) Contell C, Birnbaumer V, Flockerzi V, et al. A unified nomenclature for the superfamily of TRP cation channels. Mol. Cell 2002; 9:229-31. |
| Immunogen | TRPC3 antibody was raised against a 14 amino acid synthetic peptide from near the carboxy terminus of human TRPC3. The immunogen is located within amino acids 760 - 810 of TRPC3. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| TRPC3 antibody can be used for the detection of TRPC3 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TRPC3 |
|---|---|
| Alternative Names | TRPC3 Antibody: TRP3 TRP3 Short transient receptor potential channel 3 Transient receptor protein 3 TrpC3 |
| Sequence Similarities | 2) Cat. No. 1401 - Mouse Heart Tissue Lysate |
Application
Western blot analysis of TRPC3 in human brain tissue lysate with TRPC3 antibody at (A) 1 and (B) 2 μg/mL.
Application
Immunofluorescence of TRPC3 in Mouse Heart cells with TRPC3 antibody at 20 μg/mL.
Application
Immunohistochemistry of TRPC3 in mouse heart tissue with TRPC3 antibody at 2 μg/mL.| Positive Control | 1) Cat. No. 1303 - Human Brain Tissue Lysate |
|---|---|
| Application Notes | TRPC3 antibody can be used for the detection of TRPC3 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | TRPC3 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | TRPC3 Antibody is supplied in PBS containing 0.02% sodium azide. |
