Application
Western blot analysis of TRPC3 in human cerebellum tissue lysate with TRPC3 antibody at (A) 1 and (B) 2 μg/ml.Application
Immunohistochemistry of TRPC3 in human brain tissue with TRPC3 antibody at 2.5 μg/mL.| Product Name | TRPC3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | TRP3, TRP3, Short transient receptor potential channel 3, Transient receptor protein 3, TrpC3 |
| Product description | The mammalian transient receptor potential (TRP) superfamily can be divided into three major families including the "canonical TRP" (TRPC) family. The seven members of this family share the activation through PLC-coupled receptors and have been suggested to be components of receptor-regulated cation channels in different cell types. Furthermore, the members of the TRPC3/6/7 subfamily can be activated by diacylglycerol analogs, suggesting a possible mechanism of activation of these channels by PLC-coupled receptors. TRPC3 encodes a Ca2+-permeant channel that is agonist-activated but not store-operated or directly receptor-activated. TRPC3 physically interacts with TRPC6 and TRPC7 and forms functional tetrameric channels.1) Contell C, Birnbaumer V, Flockerzi V, et al. A unified nomenclature for the superfamily of TRP cation channels. Mol. Cell 2002; 9:229-31. |
| Immunogen | TRPC3 antibody was raised against a 19 amino acid peptide from near the carboxy terminus of human TRPC3. The immunogen is located within amino acids 710 - 760 of TRPC3. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIHC-PWB |
| TRPC3 antibody can be used for the detection of TRPC3 by Western blot at 1 - 2 μg/mL.Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TRPC3 |
|---|---|
| Alternative Names | TRP3 TRP3 Short transient receptor potential channel 3 Transient receptor protein 3 TrpC3 |
| Molecular Weight(MW) | Predicted: 90, 93, 101 kDa Observed: 93 kDa |
| Sequence Similarities | 2) Cat. No. 10-301 - Human Brain Tissue Slide |
| Tissue Specificity | TRPC3 antibody is human, mouse and rat reactive. At least four isoforms are known to exist; this antibody will detect the three largest isoforms. TRPC3 antibody is predicted to not cross-react with TRPC6. |
| Entrez Gene | 7222 |
|---|---|
| Protein Accession | NP_001124170 |
Application
Western blot analysis of TRPC3 in human cerebellum tissue lysate with TRPC3 antibody at (A) 1 and (B) 2 μg/ml.
Application
Immunohistochemistry of TRPC3 in human brain tissue with TRPC3 antibody at 2.5 μg/mL.| Positive Control | 1) Cat. No. 1362 - Human Cerebellum Tissue Lysate |
|---|---|
| Application Notes | TRPC3 antibody can be used for the detection of TRPC3 by Western blot at 1 - 2 μg/mL.Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | TRPC3 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | TRPC3 Antibody is supplied in PBS containing 0.02% sodium azide. |
