Application
Western blot analysis of TRPC6 in mouse lung tissue lysate with TRPC6 antibody at (A) 0.5, (B) 1 and (C) 2 μg/mL.Application
Immunofluorescence of TRPC6 in Mouse Lung cells with TRPC6 antibody at 20 μg/mL.Application
Immunohistochemistry of TRPC6 in mouse lung tissue with TRPC6 antibody at 5 μg/ml.Application
Immunohistochemistry of TRPC6 in mouse lung tissue with TRPC6 antibody at 10 μg/mL.| Product Name | TRPC6 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | TRPC6 Antibody: The mammalian transient receptor potential (TRP) superfamily can be divided into three major families including the "canonical TRP" (TRPC) family. The seven members of this family share the activation through PLC-coupled receptors and have been suggested to be components of receptor-regulated cation channels in different cell types. Furthermore, the members of the TRPC6/6/7 subfamily can be activated by diacylglycerol (DAG) analogs, suggesting a possible mechanism of activation of these channels by PLC-coupled receptors. When expressed in transfected cells, TRPC6 acts as a non-selective store-independent receptor-activated cation channel. It is activated by DAG in a PKC-independent manner and is insensitive to IP3 activation. There is increasing evidence that TRPC6 encodes endogenous DAG-activated receptor-operated cation channels in vivo.1) Contell C, Birnbaumer V, Flockerzi V, et al. A unified nomenclature for the superfamily of TRP cation channels. Mol. Cell 2002; 9:229-31. |
| Immunogen | TRPC6 antibody was raised against a 14 amino acid synthetic peptide from near the carboxy terminus of human TRPC6. The immunogen is located within the last 50 amino acids of TRPC6. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| TRPC6 antibody can be used for the detection of TRPC6 by Western blot at 0.5 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TRPC6 |
|---|---|
| Alternative Names | TRPC6 Antibody: TRP6 FSGS2 TRP6 Short transient receptor potential channel 6 Transient receptor protein 6 TrpC6 |
| Molecular Weight(MW) | Predicted: 100, 102 kDa Observed: 93 kDa |
| Tissue Specificity | This antibody may cross-react with TRPC3. |
Application
Western blot analysis of TRPC6 in mouse lung tissue lysate with TRPC6 antibody at (A) 0.5, (B) 1 and (C) 2 μg/mL.
Application
Immunofluorescence of TRPC6 in Mouse Lung cells with TRPC6 antibody at 20 μg/mL.
Application
Immunohistochemistry of TRPC6 in mouse lung tissue with TRPC6 antibody at 5 μg/ml.
Application
Immunohistochemistry of TRPC6 in mouse lung tissue with TRPC6 antibody at 10 μg/mL.| Positive Control | 1) Cat. No. 1402 - Mouse Lung Tissue Lysate |
|---|---|
| Application Notes | TRPC6 antibody can be used for the detection of TRPC6 by Western blot at 0.5 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | TRPC6 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | TRPC6 Antibody is supplied in PBS containing 0.02% sodium azide. |
