Application
Western blot analysis of TRPV4 in (A) human testis tissue and (B) SK-N-SH cell lysate with TRPV4 antibody at 1 μg/mL.Application
Immunofluorescence of TRPV4 in human lung tissue with TRPV4 antibody at 20 μg/mL.Application
Immunohistochemistry of TRPV4 in human lung tissue with TRPV4 antibody at 5 μg/mL.| Product Name | TRPV4 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | TRPV4 Antibody: SMAL, VRL2, CMT2C, SPSMA, TRP12, VROAC, HMSN2C, OTRPC4, SSQTL1, Transient receptor potential cation channel subfamily V member 4, Osm-9-like TRP channel 4, TrpV4 |
| Product description | The transient receptor potential (TRP) protein family consists of a diverse group of cation channels functioning in a variety of homeostatic and regulatory pathways. Four subfamilies exist, based on channel domain homology: C type (canonical), V type (vanilloid receptor related), M type (melastatin related) and P type (PKD) (1). TRPV4, belongs to the V type subfamily and plays a role in systemic osmoregulation (2,3). TRPV4 is a calcium channel multi-pass membrane protein activated by various stimuli, including thermal stress, fatty acid metabolites and hypotonicity (3). TRPV4 is highly expressed in lung and kidney and widely expressed in brain. It plays an important role in regulating neural excitability (4).1) Birnbaumer L, Yildirim E and Abramowitz J. A comparison of the genes coding for canonical TRP channels and their M, V and P relatives. Cell Calcium 2003; 33:419-32. |
| Immunogen | TRPV4 antibody was raised against an 18 amino acid peptide near the center of human TRPV4. The immunogen is located within amino acids 380 - 430 of TRPV4. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| TRPV4 antibody can be used for detection of TRPV4 by Western blot at 1 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TRPV4 |
|---|---|
| Alternative Names | TRPV4 Antibody: SMAL VRL2 CMT2C SPSMA TRP12 VROAC HMSN2C OTRPC4 SSQTL1 Transient receptor potential cation channel subfamily V member 4 Osm-9-like TRP channel 4 TrpV4 |
| Molecular Weight(MW) | Predicted: 96 kDa Observed: 100 kDa |
| Sequence Similarities | 2) Cat. No. 10-101 - Human Lung Tissue Slide |
| Tissue Specificity | TRPV4 antibody is human specific. At least six isoforms of TRPV4 are known to exist. This antibody is predicted to not cross-react with other TRP protein family members. |
Application
Western blot analysis of TRPV4 in (A) human testis tissue and (B) SK-N-SH cell lysate with TRPV4 antibody at 1 μg/mL.
Application
Immunofluorescence of TRPV4 in human lung tissue with TRPV4 antibody at 20 μg/mL.
Application
Immunohistochemistry of TRPV4 in human lung tissue with TRPV4 antibody at 5 μg/mL.| Positive Control | 1) Cat. No. 1220 - SK-N-SH Cell Lysate |
|---|---|
| Application Notes | TRPV4 antibody can be used for detection of TRPV4 by Western blot at 1 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | TRPV4 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | TRPV4 antibody is supplied in PBS containing 0.02% sodium azide. |
