Application
Tryptophan Hydroxylase (Phospho-Ser58) Antibody| Product Name | Tryptophan (phospho Ser58) Hydroxylase Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Tph, Tph, Tryptophan 5-monooxygenase 1, |
| Product description | Tryptophan hydroxylase (TPH) catalyzes the 5-hydroxylation of tryptophan, which is the first step in the biosynthesis of indoleamines (serotonin and melatonin). In mammals, serotonin biosynthesis occurs predominantly in neurons which originate in the raphe nuclei of the brain, and melatonin synthesis takes place within the pineal gland. Although TPH catalyzes the same reaction within the raphe nuclei and the pineal gland, TPH activity is rate-limiting for serotonin but not melatonin biosynthesis. Serotonin functions mainly as a neurotransmitter, whereas melatonin is the principal hormone secreted by the pineal gland. The activity of TPH is enhanced by phosphorylation by cAMP-dependent protein kinase (PKA) and Ca2+/calmodulin kinase II (CAM K II). Both PKA and CAM K II phosphorylate Ser58 which lies within the regulatory domain of TPH.1) Martinez, A., Knappskog, P.M. and Haavik, J. "A structural approach into human tryptophan hydroxylase and its implications for the regulation of serotonin biosynthesis," Curr. Med. Chem., 8 (2001) 1077 - 1091. |
| Immunogen | TPH (Ser58) polyclonal antibody was raised against a synthetic phosphopeptide corresponding to amino acid residues surrounding the phosphoSer58 of TPH. |
| Modification | p-Ser58 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | WB |
| Applications include Dot Blots (DB) and Western Blots (WB). When internally tested under ideal conditions the working dilutions were 1:1000 for DB and WB.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | Tph1 |
|---|---|
| Alternative Names | Tph Tph Tryptophan 5-monooxygenase 1 |
| Molecular Weight(MW) | 55 |
| Tissue Specificity | Tryptophan Hydroxylase antibody is specific immunolabeling of the ~55k TPH protein phosphorylated at Ser58 in Western blots of human dorsal Raphe nucleus. |
Application
Tryptophan Hydroxylase (Phospho-Ser58) Antibody| Application Notes | Applications include Dot Blots (DB) and Western Blots (WB). When internally tested under ideal conditions the working dilutions were 1:1000 for DB and WB.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For long term storage –80˚C is recommended, but shorter term storage at –20˚C is also acceptable as aliquots may be taken without freeze/thawing due to the presence of 50% glycerol. Stable for one year. |
| Storage Buffer | 100 uL in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per mL BSA and 50% glycerol. |
