Application
Western blot analysis of TSHZ3 in mouse brain tissue lysate with TSHZ3 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.Application
Immunofluorescence of TSHZ3 in mouse brain tissue with TSHZ3 antibody at 20 μg/mL.Application
Immunohistochemistry of TSHZ3 in mouse brain tissue with TSHZ3 antibody at 2.5 μg/mL.| Product Name | TSHZ3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | TSHZ3 Antibody: The Teashirt zinc finger homeobox (TSHZ) family comprise a family of evolutionarily conserved transcription factors that, in Drosophila, are active in specific body parts for patterning, but whose function in vertebrates is less clear. In mice, the known three TSHZ proteins are expressed in distinct patterns in the developing and adult brain, suggesting that they play a role in the establishment of regional identity and specification of cell types within the brain. Both TSHZ3 and TSHZ1 have been found to interact with FE65, an adapter protein that binds to the amyloid protein precursor (APP) in neurons. Together with SET, a component of the inhibitor of acetyl transferase, and histone deacetylases, these proteins formed a gene-silencing complex whose target includes caspase-4. Recent experiments have also suggested this family of proteins may be involved in carcinogenesis.1) Caubit X, Core N, Boned A, et al. Vertebrate orthologues of the Drosophila region-specific patterning gene teashirt. Mech. Dev. 2000; 91:445-8. |
| Immunogen | TSHZ3 antibody was raised against an 18 amino acid synthetic peptide near the amino terminus of human TSHZ3. The immunogen is located within amino acids 60 - 110 of TSHZ3. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| TSHZ3 antibody can be used for detection of TSHZ3 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 2.5 μg/mL. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TSHZ3 |
|---|---|
| Alternative Names | TSHZ3 Antibody: TSH3 ZNF537 KIAA1474 TSH3 Teashirt homolog 3 Zinc finger protein 537 |
Application
Western blot analysis of TSHZ3 in mouse brain tissue lysate with TSHZ3 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Application
Immunofluorescence of TSHZ3 in mouse brain tissue with TSHZ3 antibody at 20 μg/mL.
Application
Immunohistochemistry of TSHZ3 in mouse brain tissue with TSHZ3 antibody at 2.5 μg/mL.| Positive Control | 1) Cat. No. 1403 - Mouse Brain Tissue Lysate |
|---|---|
| Application Notes | TSHZ3 antibody can be used for detection of TSHZ3 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 2.5 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | TSHZ3 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | TSHZ3 Antibody is supplied in PBS containing 0.02% sodium azide. |
