Application
Western blot analysis of TSPAN7 Antibody in CEM cell line lysates (35ug/lane)Application
Flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | TSPAN7 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Tetraspanin-7, Tspan-7, Cell surface glycoprotein A15, Membrane component chromosome X surface marker 1, T-cell acute lymphoblastic leukemia-associated antigen 1, TALLA-1, Transmembrane 4 superfamily member 2, CD231, TSPAN7, A15, DXS1692E, MXS1, TM4SF2 |
| Product description | TSPAN7 is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. This encoded protein is a cell surface glycoprotein and may have a role in the control of neurite outgrowth. It is known to complex with integrins. This gene is associated with X-linked mental retardation and neuropsychiatric diseases such as Huntington's chorea, fragile X syndrome and myotonic dystrophy.1) Radhakrishnan, Y., et al. Biol. Reprod. 81(4):647-656(2009) |
| Immunogen | This TSPAN7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 98-124 amino acids from the Central region of human TSPAN7. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSWB |
| For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TSPAN7 |
|---|---|
| Alternative Names | Tetraspanin-7 Tspan-7 Cell surface glycoprotein A15 Membrane component chromosome X surface marker 1 T-cell acute lymphoblastic leukemia-associated antigen 1 TALLA-1 Transmembrane 4 superfamily member 2 CD231 TSPAN7 A15 DXS1692E MXS1 TM4SF2 |
| Molecular Weight(MW) | 28 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Mouse |
Application
Western blot analysis of TSPAN7 Antibody in CEM cell line lysates (35ug/lane)
Application
Flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
