Application
Western blot analysis of TSPY1S in A20 cell lysate with TSPY1S antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.| Product Name | TSPY1S Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | TSPY1S Antibody: CT78, TSPY, DYS14, pJA923, Testis-specific Y-encoded protein 1, CT78 |
| Product description | TSPY1S Antibody: Testis-specific protein on Y chromosome (TSPY1) is an ampliconic gene on the Y chromosome that has been associated with gonadoblastoma. Recent experiments have shown that in androgen-dependent testicular germ-cell tumors, TSPY1 can repress the androgen-bound androgen receptor (AR), a member of the nuclear steroid hormone receptor family that acts as a ligand-inducible transcription factor, suggesting that TSPY1 is a repressor of cell proliferation in germ-cell tumors and potentially in normal gonadal cells during early development. Two distinct isoforms of TSPY1, TSPY1L and TSPY1S, are known to exist.1) Arnemann J, Jakubiczka S, Thuring S, et al. Cloning and sequence analysis of a human Y-chromosome-derived, testicular cDNA, TSPY. Genomics 1991; 11:108-114 |
| Immunogen | Rabbit polyclonal TSPY1S antibody was raised against a 13 amino acid peptide near the carboxy terminus of human TSPY1S. The immunogen is located within the last 50 amino acids of TSPY1S. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| TSPY1S antibody can be used for detection of TSPY1S by Western blot at 1 - 2 μg/mL.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TSPY1 |
|---|---|
| Alternative Names | TSPY1S Antibody: CT78 TSPY DYS14 pJA923 Testis-specific Y-encoded protein 1 CT78 |
| Molecular Weight(MW) | 32 kDa |
| Tissue Specificity | TSPY1S antibody is human and mouse reactive. At least three isoforms of TSPY1 are known to exist; this antibody will detect only TSPY1S |
| Entrez Gene | 7258 |
|---|---|
| Protein Accession | NP_001184171 |
Application
Western blot analysis of TSPY1S in A20 cell lysate with TSPY1S antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.| Positive Control | 1) Cat. No. 1288 - A20 Cell Lysate |
|---|---|
| Application Notes | TSPY1S antibody can be used for detection of TSPY1S by Western blot at 1 - 2 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | TSPY1S antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | TSPY1S Antibody is supplied in PBS containing 0.02% sodium azide. |
