Application
Western blot analysis of TTBK1 in Jurkat lysate with TTBK1 antibody at 1 μg/mL.Application
Immunofluorescence of TTBK1 in Human Brain tissue with TTBK1 antibody at 20 μg/mL.Application
Immunohistochemistry of TTBK1 in mouse brain tissue with TTBK1 antibody at 2.5 μg/mL.| Product Name | TTBK1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | TTBK1 Antibody: BDTK, BDTK, KIAA1855, Tau-tubulin kinase 1, Brain-derived tau kinase |
| Product description | TTBK1 Antibody: Tau tubulin kinase (TTBK1) belongs to the casein kinase 1 superfamily and is involved in the phosphorylation of specific serine/threonine residues in paired helical filaments of the tau protein. It is specifically expressed in the brain and induces tau aggregation in human neuronal cells in a dose-dependent manner. TTBK1 levels have been found to be upregulated in the brains of Alzheimer's disease (AD) patients, and mice expressing human TTBK1 protein showed significant age-dependent memory impairment. These mice displayed increased levels of the CDK5 activators p25 and p35, and reduced levels of the NMDA receptor types 2B and 2D, suggesting that TTBK1 may play a role in memory dysfunction in AD patients.1) Sato S, Cerny RL, Bueschner JL, et al. Tau-tubulin kinase 1 (TTBK1), a neuron-specific tau kinase candidate, is involved in tau phosphorylation and aggregation. J. Neurochem. 2006; 98:1573-84. |
| Immunogen | TTBK1 antibody was raised against a 16 amino acid synthetic peptide near the carboxy terminus of human TTBK1. The immunogen is located within amino acids 1190 - 1240 of TTBK1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| TTBK1 antibody can be used for detection of TTBK1 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TTBK1 |
|---|---|
| Alternative Names | TTBK1 Antibody: BDTK BDTK KIAA1855 Tau-tubulin kinase 1 Brain-derived tau kinase |
| Molecular Weight(MW) | Predicted: 145, 151 kDa Observed: 140 kDa |
| Sequence Similarities | 2) Cat. No. 10-301 - Human Brain Tissue Slide |
| Tissue Specificity | Multiple isoforms of TTBK1 are known to exist. This antibody is predicted to not cross-react with TTBK2. |
Application
Western blot analysis of TTBK1 in Jurkat lysate with TTBK1 antibody at 1 μg/mL.
Application
Immunofluorescence of TTBK1 in Human Brain tissue with TTBK1 antibody at 20 μg/mL.
Application
Immunohistochemistry of TTBK1 in mouse brain tissue with TTBK1 antibody at 2.5 μg/mL.| Positive Control | 1) Cat. No. 1205 - Jurkat Cell Lysate |
|---|---|
| Application Notes | TTBK1 antibody can be used for detection of TTBK1 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | TTBK1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | TTBK1 Antibody is supplied in PBS containing 0.02% sodium azide. |
