Application
Western blot analysis of TTC5 in RAW264.7 cell lysate with TTC5 antibody at (A) 0.5, (B) 1 and (C) 2 μg/mL.Application
Immunofluorescence of TTC5 in Mouse Brain cells with TTC5 antibody at 20 μg/mL.Application
Immunohistochemistry of TTC5 in mouse brain tissue with TTC5 antibody at 10 μg/mL.WB
Expressions of TRIP13, TTC5, p-p53, N-cadherin and E-cadherin in thyroid cancer (TC) and normal tissues which were determined by RT-PCR (A),WB
and western blot (C).WB
mRNA (A) and proteinWB
(B) expressions of TTC5, N-cadherin and E-cadherin in TPC1 and BHT101 cells transfected with sh-TRIP13 and empty vector which were evaluated by RT-PCR and western blot, respectively.IHC
immunohistochemical analysis (B)| Product Name | TTC5 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | TTC5 Antibody: Tetratricopeptide repeat protein 5 (TTC5) is a member of a diverse group of functionally distinct proteins that are characterized by containing one or more tetratricopeptide repeats. Each motif consists of two anti-parallel a-helices such that tandem arrays of TPR motifs generate a right-handed helical structure with an amphipathic channel that may serve to accommodate the complementary region of a target protein. While the exact function of TTC5 remains unclear, it is thought that the TPR motifs serve to mediate protein-protein interactions such as those seen with protein chaperones HSP70 and HSP90 and some proteins involved in cell stress response signaling pathways such as protein phosphatase 5, suggesting that TTC5 may also function via protein-protein interactions mediated by its TPR motifs.1) Blatch GL and Lassle M. The tetratricopeptide repeat: a structural motif mediating protein-protein interactions. BioEssays 1999; 21:932-9 |
| Immunogen | TTC5 antibody was raised against a 14 amino acid synthetic peptide from near the carboxy terminus of human TTC5. The immunogen is located within the last 50 amino acids of TTC5. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| TTC5 antibody can be used for the detection of TTC5 by Western blot at 0.5 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TTC5 |
|---|---|
| Alternative Names | TTC5 Antibody: Strap Tetratricopeptide repeat protein 5 Stress-responsive activator of p300 TPR repeat protein 5 |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Rat: (100%), Bovine: (93%) |
Application
Western blot analysis of TTC5 in RAW264.7 cell lysate with TTC5 antibody at (A) 0.5, (B) 1 and (C) 2 μg/mL.
Application
Immunofluorescence of TTC5 in Mouse Brain cells with TTC5 antibody at 20 μg/mL.
Application
Immunohistochemistry of TTC5 in mouse brain tissue with TTC5 antibody at 10 μg/mL.
WB
Expressions of TRIP13, TTC5, p-p53, N-cadherin and E-cadherin in thyroid cancer (TC) and normal tissues which were determined by RT-PCR (A),
WB
and western blot (C).
WB
mRNA (A) and protein
WB
(B) expressions of TTC5, N-cadherin and E-cadherin in TPC1 and BHT101 cells transfected with sh-TRIP13 and empty vector which were evaluated by RT-PCR and western blot, respectively.
IHC
immunohistochemical analysis (B)| Positive Control | 1) Cat. No. 1283 - RAW 264.7 Cell Lysate |
|---|---|
| Application Notes | TTC5 antibody can be used for the detection of TTC5 by Western blot at 0.5 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | TTC5 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | TTC5 Antibody is supplied in PBS containing 0.02% sodium azide. |
