Application
Western blot analysis in Ramos cell line lysates (35ug/lane).| Product Name | TTL Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Tubulin--tyrosine ligase, TTL, TTL |
| Product description | TTL is a cytosolic enzyme involved in the posttranslational modification of alpha-tubulin (see MIM 602529). Alpha-tubulin within assembled microtubules is detyrosinated over time at the C terminus. After microtubule disassembly, TTL restores the tyrosine residues and consequently participates in a cycle of tubulin detyrosination and tyrosination (Erck et al., 2003 [PubMed 14571137]).1) Dick, D.M., et al. Am. J. Med. Genet.Neuropsychiatr. Genet. 153B (6), 1179-1188 (2010) : |
| Immunogen | This TTL antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 232-263 amino acids from the C-terminal region of human TTL. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB starting dilution is: 1:1000: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TTL |
|---|---|
| Alternative Names | Tubulin--tyrosine ligase TTL TTL |
| Molecular Weight(MW) | 43 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine |
Application
Western blot analysis in Ramos cell line lysates (35ug/lane).| Application Notes | For WB starting dilution is: 1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
