Application
Western blot analysis of extracts from Rat brain cells using TUBA1/3/4 (Phospho-Tyr272) Antibody. The lane on the right is treated with the antigen-specific peptide.| Product Name | TUBA1/3/4 (phospho Tyr272) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | TBA1, TBA1A, TBA4A, TUBA1 |
| Product description | Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulins. The genes encoding these microtubule constituents belong to the tubulin superfamily, which is composed of six distinct families. Genes from the alpha, beta and gamma tubulin families are found in all eukaryotes. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes, which are highly conserved among species.1) Adler A.J., Bioorg. Med. Chem. 9:1967-1976(2001). |
| Immunogen | TUBA1/3/4 (phospho Tyr272) antibody was raised against a peptide sequence around phosphorylation site of tyrosine 272 (A-T-Y (p)-A-P) derived from Human TUBA1/3/4. |
| Modification | p-Tyr272 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| Western Blot: 1:500~1:1000: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TUBA1A, TUBA1B, TUBA1C |
|---|---|
| Alternative Names | TBA1 TBA1A TBA4A TUBA1 |
| Molecular Weight(MW) | 50-55 kDa |
| Tissue Specificity | The antibody detects endogenous levels of TUBA1/3/4 only when phosphorylated at tyrosine 272. |
| Entrez Gene | 7846/10376/84790/113457/7278/112714/7277 |
|---|---|
| Protein Accession | NP_001257328.1 |
Application
Western blot analysis of extracts from Rat brain cells using TUBA1/3/4 (Phospho-Tyr272) Antibody. The lane on the right is treated with the antigen-specific peptide.| Application Notes | Western Blot: 1:500~1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
