Application
Western blot analysis of TWEAK in HeLa cell lysate with TWEAK antibody at (A) 1 and (B) 2 μg/mL.Application
Immunohistochemistry of TWEAK in mouse brain tissue with TWEAK antibody at 5 μg/mL.Application
Immunohistochemistry of TWEAK in human brain tissue with TWEAK antibody at 10 μg/mL.| Product Name | TWEAK Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | TWEAK Antibody: APO3L, DR3LG, TWEAK, APO3L, UNQ181/PRO207, Tumor necrosis factor ligand superfamily member 12, APO3 ligand |
| Product description | TWEAK Antibody: TNF-related weak inducer of apoptosis (TWEAK) is a member of the tumor necrosis factor superfamily (TNFSF) of structurally related cytokines. Like most other members of this family, TWEAK is a cell surface-associated type II transmembrane protein although a smaller, biologically active form can also be generated by cleavage near the cell membrane. TWEAK has multiple biological activities, including stimulation of cell growth and angiogenesis, induction of inflammatory cytokines, in addition to stimulation of apoptosis. The TWEAK signal transduction pathway has not been well established but it appears to signal via TweakR/Fn14 in a manner similar to that described for other TNFSF members that bind receptors lacking death domains.1) Wiley SR and Winkles JA. TWEAK, a member of the TNF superfamily, is a multifunctional cytokine that binds the TweakR/Fn14 receptor. Cyto. And Growth Factor Rev. 2003; 14:241-9. |
| Immunogen | TWEAK antibody was raised against recombinant human TWEAK protein. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIHC-PWB |
| TWEAK antibody can be used for the detection of TWEAK by Western blot at 1 to 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | TNFSF12 |
|---|---|
| Alternative Names | TWEAK Antibody: APO3L DR3LG TWEAK APO3L UNQ181/PRO207 Tumor necrosis factor ligand superfamily member 12 APO3 ligand |
| Molecular Weight(MW) | Predicted: 27 kDa Observed: 32 kDa |
| Sequence Similarities | 3) Cat. No. 10-301 - Human Brain Tissue Slide |
Application
Western blot analysis of TWEAK in HeLa cell lysate with TWEAK antibody at (A) 1 and (B) 2 μg/mL.
Application
Immunohistochemistry of TWEAK in mouse brain tissue with TWEAK antibody at 5 μg/mL.
Application
Immunohistochemistry of TWEAK in human brain tissue with TWEAK antibody at 10 μg/mL.| Positive Control | 1) Cat. No. 1303 - Human Brain Tissue Lysate |
|---|---|
| Application Notes | TWEAK antibody can be used for the detection of TWEAK by Western blot at 1 to 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | TWEAK antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | TWEAK Antibody is supplied in PBS containing 0.02% sodium azide. |
